Development and Application of an Indirect Enzyme-Linked Immunosorbent Assay Based on a Recombinant Matrix Protein for the Serological Study of Porcine Deltacoronavirus in Mexican Pigs

Overview

Journal Vet Med Sci

Specialty Veterinary Medicine

Date 2024 Nov 4

PMID 39494986

Authors

Francisco Jesus Castaneda Montes

Jose Luis Cerriteno Sanchez

Julieta Sandra Cuevas-Romero

Maria Azucena Castaneda Montes

Susana Mendoza Elvira

Affiliations

Soon will be listed here.

Abstract

Porcine deltacoronavirus (PDCoV) is an infectious disease that causes diarrhoea in pigs of different ages; however, piglets are more susceptible. PDCoV was first reported in 2012 in China and Hong Kong. Later, it was first reported in the USA in 2014 and in Mexico in 2019. Several studies have shown that M protein is highly conserved and, therefore, suitable for diagnostic systems. In this study, for the first time, an indirect enzyme-linked immunosorbent assay (iELISA) based on a recombinant M protein (rM-PDCoV) was developed to evaluate the seroprevalence of PDCoV in four states in Mexico. High sensitivity (83%) and specificity (100%) were observed for the iELISA. The kappa index calculated a nearly perfect agreement (0.8831) compared to the Western blot (gold standard test), suggesting acceptable statistical value support. In this study, 50.38% of the serum samples from backyard pigs were PDCoV-positive. The serological comparison showed that PDCoV/PEDV coinfections occurred in 31.98% of the analysed sera. These results can enrich our understanding of how this virus spreads and enable the evaluation of PDCoV infections. Moreover, it highlights the importance of continually investigating the seroprevalence of PDCoV in Mexico because there is also no information about the current prevalence of the disease.

Citing Articles

Seroepidemiology for in Mexican Pigs by Development of an Indirect ELISA Based on a Recombinant NP Protein.

Lara-Romero R, Cerriteno-Sanchez J, Castaneda-Montes M, Ramirez-Mendoza H, Cuevas-Romero J Pathogens. 2025; 13(12.

PMID: 39770394 PMC: 11677145. DOI: 10.3390/pathogens13121135.

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