Urinary Health Indicators in Folivorous-frugivorous Primates with and Without Food Supplementation

Overview

Journal Primates

Specialty Biology

Date 2024 Nov 1

PMID 39485588

Authors

Janaina Paula Back

Julio Cesar Bicca-Marques

Affiliations

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Abstract

Food supplementation by humans in peri-urban and urban landscapes can lead to excessive intake of energy and certain macronutrients, and affect animal health. In this study, we evaluated the influence of food supplementation on urinary health indicators in brown howler monkeys (Alouatta guariba) by comparing supplemented and non-supplemented free-ranging peri-urban groups. We also evaluated the effect of sex, day shift, and season of sampling. Between August 2021 and August 2022, we non-invasively collected 61 samples (43 from females and 18 from males) from adult individuals (N = 10) in three supplemented groups and 56 samples (25 from females and 31 from males) from adults (N = 7) in three non-supplemented groups. The supplements, mostly raw foods (e.g., fruits, vegetables, and tubers) and bread, represented 18% of the total fresh mass ingested by the supplemented groups. We assessed pH, density, and the presence of eight urine components (glucose, bilirubin, ketones, protein, urobilinogen, nitrite, blood, and leukocytes) using reagent urine strips. Season of sampling predicted urine density (mean = 1.022), while both season and day shift predicted pH (mean = 6.5). The occurrence of supplementation was a weak predictor of these parameters. Finally, we detected leukocytes in 21% of the 117 samples. We did not identify any visible signs of disease in any individual throughout the study and found no clinical changes in urine under the conditions studied. We urge validation of the results with urine strips to facilitate monitoring of the health of howler monkeys living in anthropogenic landscapes in the presence or absence of dietary supplementation.

References

Altmann J . Observational study of behavior: sampling methods. Behaviour. 1974; 49(3):227-67. DOI: 10.1163/156853974x00534. View

Banks W, Altmann J, Sapolsky R, Phillips-Conroy J, Morley J . Serum leptin levels as a marker for a syndrome X-like condition in wild baboons. J Clin Endocrinol Metab. 2003; 88(3):1234-40. DOI: 10.1210/jc.2002-021695. View

Barghouthy Y, Somani B . Role of Citrus Fruit Juices in Prevention of Kidney Stone Disease (KSD): A Narrative Review. Nutrients. 2021; 13(11). PMC: 8625077. DOI: 10.3390/nu13114117. View

Beaman B, Hesemeyer W, Dominy N, Savini T, Reichard U . Sterile pyuria in a population of wild white-handed gibbons (Hylobates lar). Am J Primatol. 2009; 71(10):880-3. DOI: 10.1002/ajp.20718. View

Blanco G, Lemus J, Garcia-Montijano M . When conservation management becomes contraindicated: impact of food supplementation on health of endangered wildlife. Ecol Appl. 2011; 21(7):2469-77. DOI: 10.1890/11-0038.1. View

Cameron M, Maalouf N, Poindexter J, Adams-Huet B, Sakhaee K, Moe O . The diurnal variation in urine acidification differs between normal individuals and uric acid stone formers. Kidney Int. 2012; 81(11):1123-30. PMC: 3352978. DOI: 10.1038/ki.2011.480. View

Chaves O, Bicca-Marques J . Dietary flexibility of the brown howler monkey throughout its geographic distribution. Am J Primatol. 2012; 75(1):16-29. DOI: 10.1002/ajp.22075. View

Chaves O, Bicca-Marques J . Feeding Strategies of Brown Howler Monkeys in Response to Variations in Food Availability. PLoS One. 2016; 11(2):e0145819. PMC: 4743924. DOI: 10.1371/journal.pone.0145819. View

Chaves O, Fernandes F, Oliveira G, Bicca-Marques J . Assessing the influence of biotic, abiotic, and social factors on the physiological stress of a large Neotropical primate in Atlantic forest fragments. Sci Total Environ. 2019; 690:705-716. DOI: 10.1016/j.scitotenv.2019.07.033. View

10.

Chaves O, Fortes V, Hass G, Azevedo R, Stoner K, Bicca-Marques J . Flower consumption, ambient temperature and rainfall modulate drinking behavior in a folivorous-frugivorous arboreal mammal. PLoS One. 2021; 16(2):e0236974. PMC: 7894884. DOI: 10.1371/journal.pone.0236974. View

11.

Chaves O, Junior J, Buss G, Hirano Z, Jardim M, Amaral E . Wildlife is imperiled in peri-urban landscapes: threats to arboreal mammals. Sci Total Environ. 2022; 821:152883. DOI: 10.1016/j.scitotenv.2021.152883. View

12.

Culot L, Pereira L, Agostini I, Barreto de Almeida M, Alves R, Aximoff I . ATLANTIC-PRIMATES: a dataset of communities and occurrences of primates in the Atlantic Forests of South America. Ecology. 2018; 100(1):e02525. DOI: 10.1002/ecy.2525. View

13.

Dieter R . Sterile pyuria: a differential diagnosis. Compr Ther. 2000; 26(3):150-2. DOI: 10.1007/s12019-000-0001-1. View

14.

Grueber C, Nakagawa S, Laws R, Jamieson I . Multimodel inference in ecology and evolution: challenges and solutions. J Evol Biol. 2011; 24(4):699-711. DOI: 10.1111/j.1420-9101.2010.02210.x. View

15.

Heilberg I, Schor N . [Diagnosis and clinical management of urinary tract infection]. Rev Assoc Med Bras (1992). 2003; 49(1):109-16. DOI: 10.1590/s0104-42302003000100043. View

16.

Hekmatynia F, Eskandarzadeh N, Imani M, Rezaei M, Zamani-Ahmadmahmudi M . The diagnostic performance of human urinary dipsticks to estimate urine pH, specific gravity (SpG), and protein in horses: are they reliable?. BMC Vet Res. 2019; 15(1):242. PMC: 6626356. DOI: 10.1186/s12917-019-1998-2. View

17.

Karasu G, Rogers C, Gee E . Dietary Transitions Toward Sustainable Horse Feeding. J Equine Vet Sci. 2023; 128:104880. DOI: 10.1016/j.jevs.2023.104880. View

18.

Kaur T, Huffman M . Descriptive urological record of chimpanzees (Pan troglodytes) in the wild and limitations associated with using multi-reagent dipstick test strips. J Med Primatol. 2004; 33(4):187-96. DOI: 10.1111/j.1600-0684.2004.00070.x. View

19.

Kavouras S . Assessing hydration status. Curr Opin Clin Nutr Metab Care. 2002; 5(5):519-24. DOI: 10.1097/00075197-200209000-00010. View

20.

Kelly T, Sleeman J, Wrangham R . Urinalysis in free-living chimpanzees (Pan troglodytes schweinfurthii) in Uganda. Vet Rec. 2004; 154(23):729-30. DOI: 10.1136/vr.154.23.729. View