An Interkinetic Envelope Surrounds Chromosomes Between Meiosis I and II in Oocytes

Overview

Journal bioRxiv

Date 2024 Nov 1

PMID 39484525

Authors

Layla El Mossadeq

Laura Bellutti

Remi Le Borgne

Julie C Canman

Lionel Pintard

Jean-Marc Verbavatz

Peter Askjaer

Julien Dumont

Affiliations

Soon will be listed here.

Abstract

At the end of cell division, the nuclear envelope reassembles around the decondensing chromosomes. Female meiosis culminates in two consecutive cell divisions of the oocyte, meiosis I and II, which are separated by a brief transition phase known as interkinesis. Due to the absence of chromosome decondensation and the suppression of genome replication during interkinesis, it has been widely assumed that the nuclear envelope does not reassemble between meiosis I and II. By analyzing interkinesis in oocytes, we instead show that an atypical structure made of two lipid bilayers, which we termed the interkinetic envelope, surrounds the surface of the segregating chromosomes. The interkinetic envelope shares common features with the nuclear envelope but also exhibits specific characteristics that distinguish it, including its lack of continuity with the endoplasmic reticulum, unique protein composition, assembly mechanism, and function in chromosome segregation. These distinct attributes collectively define the interkinetic envelope as a unique and specialized structure that has been previously overlooked.

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