Optimized Full-spectrum Flow Cytometry Panel for Deep Immunophenotyping of Murine Lungs

Overview

Journal Cell Rep Methods

Specialty Cell Biology

Date 2024 Oct 31

PMID 39481389

Authors

Zora Baumann

Carsten Wiethe

Cinja M Vecchi

Veronica Richina

Telma Lopes

Mohamed Bentires-Alj

Affiliations

Soon will be listed here.

Abstract

The lung immune system consists of both resident and circulating immune cells that communicate intricately. The immune system is activated by exposure to bacteria and viruses, when cancer initiates in the lung (primary lung cancer), or when metastases of other cancer types, including breast cancer, spread to and develop in the lung (secondary lung cancer). Thus, in these pathological situations, a comprehensive and quantitative assessment of changes in the lung immune system is of paramount importance for understanding mechanisms of infectious diseases, lung cancer, and metastasis but also for developing efficacious treatments. Unfortunately, lung tissue exhibits high autofluorescence, and this high background signal makes high-parameter flow cytometry analysis complicated. Here, we provide an optimized 30-parameter antibody panel for the analysis of all major immune cell types and states in normal and metastatic murine lungs using spectral flow cytometry.

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