Antimicrobial Properties of Newer Calcium Silicate-Based Pulp-Capping Agents Against Enterococcus Faecalis and Streptococcus Mutans: An In-Vitro Evaluation

Overview

Journal Cureus

Specialty Biomedical Engineering

Date 2024 Oct 31

PMID 39479088

Authors

Niranjana M

Sunil Jose

George Thomas

Arun Shyam

Aparna M

Affiliations

Soon will be listed here.

Abstract

Aim: This study aimed to evaluate the antibacterial activity of calcium silicate-based pulp-capping agents against and using an agar diffusion test.

Methods And Materials: The agar diffusion method was used to evaluate the antibacterial properties of pulp-capping agents. The materials used included Bio-C® Temp (Angelus, Brazil), Dia-Root™ Bio mineral trioxide aggregate (MTA) (Diadent Europe B.V., Almere, Netherlands), Biodentine™ (Septodont, Saint-Maur-des-Fossés, France), and TheraCal LC (Bisco Inc., Schaumburg, IL). Eighteen petri dishes, nine for and nine for , were divided into four parts each (one for each agent), for a total sample size of 72. The bacterial suspensions were transferred to the petri dishes using a sterile swab. Four wells with a diameter of 4 mm were then punched in each petri dish. The wells were filled with the pulp-capping agents, which had been mixed according to the manufacturer's instructions, and the petri dishes were incubated. The zone of inhibition was measured at 24 and 48 hours to assess the pulp-capping agents' antimicrobial efficacy against and . The readings were tabulated and subjected to statistical analysis.

Results: At 24 hours, the highest zone of inhibition was found in the Biodentine™ group (15.83 ± 0.79 mm), followed by Dia-Root™ Bio MTA (14.5 ± 0.88 mm), TheraCal LC® (12.56 ± 0.53 mm), and the shortest in the Bio-C Temp (9.61 ± 0.70 mm) against . Analysis of variance (ANOVA) test showed a high statistical significance. After 48 hours, there was no statistically significant difference in the mean zone of inhibition. At 24 hours, the highest zone of inhibition was found in the Biodentine™ group (20.56 ± 0.73 mm), followed by Dia-Root™ Bio MTA (20.06 ± 1.33 mm), TheraCal LC® (18.22 ± 0.97 mm), and the shortest in the Bio-C Temp (14.11 ± 0.78 mm) against The ANOVA test indicated no statistically significant difference between the Biodentine™ and the Dia-Root™ Bio MTA groups. After 48 hours, there was no statistically significant difference in the mean zone of inhibition.

Conclusions: Biodentine™ has higher antibacterial efficacy against , while Biodentine™ and Dia-Root™ Bio MTA have comparably high antibacterial activity against and at 24 and 48 hours.

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