Crucial Immunological Roles of the Invasion Front in Innate and Adaptive Immunity in Cervical Cancer

Overview

Journal Br J Cancer

Specialty Oncology

Date 2024 Oct 30

PMID 39472714

Authors

Yuhya Hirahara

Kanako Shimizu

Satoru Yamasaki

Tomonori Iyoda

Shogo Ueda

Shinya Sato

Jotaro Harada

Haruya Saji

Satoshi Fujii

Yohei Miyagi

Etsuko Miyagi

Shin-Ichiro Fujii

Affiliations

Soon will be listed here.

Abstract

Background: The immunostimulatory actions of innate and adaptive immune responses play a crucial role in the cancer-immunity cycle. Although cervical cancer (CC) exhibits a high recurrence rate, the relation with lymphocytes in the tumor tissue have not been analyzed.

Methods: We analyzed NKT, NK, and T cells, not only in peripheral blood (PB), but also tumor tissue through histological analysis from 23 patients with CC collected before treatment. A correlation of them between PB and the tumor tissue were assessed.

Results: We detected functional NKT and NKG2D NK cells and effector CD4 Tregs in PB. In the tumor, we detected the infiltration of LAG-3 TIM-3 CD4 and CD8 T cells rather than NK cells particularly in the invasion front (IF) by fluorescent multiplex immunohistochemistry. The heatmap and correlation analysis revealed that LAG-3 TIM-3 CD8 T cells are highly associated with CD69 CD103 exhausted CD8 T cells. We identified the statistical relationship between CD4Tregs in PB and the number of LAG-3 TIM-3 CD4 T cells in the IF, which may be related to recurrence in patients with CC.

Conclusions: LAG-3 TIM-3 T cells located in the IF may play a key role in regulation of the tumor immune microenvironment.

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