Preparation of D-NCCDs and Its Application in Fluorescent/colorimetric Dual-mode Discrimination of Glutamine Enantiomers

Overview

Journal Mikrochim Acta

Specialties Biotechnology
Chemistry

Date 2024 Oct 29

PMID 39467880

Authors

Xuan Liao

Cancan Lu

Yaning Duan

Cuiling Ren

Hongli Chen

Affiliations

Soon will be listed here.

Abstract

A new type of carbon dots (D-NCCDs) was synthesized by 3, 5-diaminobenzoic acid, N,N-dimethyl-o-phenylenediamine, and D-cysteine. The morphology and structure of D-NCCDs were investigated by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and FT-IR spectra, and the chirality was characterized by circular dichroism. In the presence of hydrogen peroxide, the fluorescence of D-NCCDs at 487 nm (λ = 410 nm) showed great discrimination ability towards glutamine enantiomers. The ratio of fluorescence intensity (F/F) to the concentration of D-Gln showed good linearity in the range 0.5-10 mM, with a detection limits of 0.11 mM. Meanwhile, the color of the solution gradually changed from light yellow to yellow-brown. The UV-Vis absorption ratio (A/A) at 410 nm showed good linearity with the concentration of D-Gln in the range 0.5 to 20 mM; the detection limit is 7.7 μM. But the fluorescence and absorbance of D-NCCDs showed no significant change after the addition of L-glutamine. Thus, fluorescence and colorimetry dual-mode discrimination of glutamine enantiomers was achieved. The fluorescence enantioselectivity of Gln (F/F) is 1.62, and the colorimetric enantioselectivity of Gln (A/A) is 2.14. The chiral discrimination mechanism of D-NCCDs to Gln enantiomers was also investigated systematically. This work not only can discriminate glutamine enantiomers with high sensitivity and convenience, but also offers a new strategy for preparing new dual mode chiral nanoprobes.

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