Hybrid Raman and Partial Wave Spectroscopy Microscope for the Characterization of Molecular and Structural Alterations in Tissue

Overview

Journal J Biophotonics

Date 2024 Oct 27

PMID 39462506

Authors

Elena Kriukova

Mikhail Mazurenka

Sabrina Marcazzan

Sarah Glasl

Michael Quante

Dieter Saur

Markus Tschurtschenthaler

Gerwin J Puppels

Dimitris Gorpas

Vasilis Ntziachristos

Affiliations

Soon will be listed here.

Abstract

We present a hybrid Raman spectroscopy (RS) and partial wave spectroscopy (PWS) microscope for the characterization of molecular and structural tissue alterations. The PWS performance was assessed with surface roughness standards, while the Raman performance with a silicon crystal standard. We also validated the system on stomach and intestinal mouse tissues, two closely-related tissue types, and demonstrate that the addition of PWS information improves RS data classification for these tissue types from R = 0.892 to R = 0.964 (norm of residuals 0.863 and 0.497, respectively). Then, in a proof-of-concept experiment, we show that the hybrid system can detect changes in intestinal tissues harvested from a tumorigenic Villin-Cre, Apc mouse. We discuss how the hybrid modality offers new abilities to identify the relative roles of PWS morphological features and Raman molecular fingerprints, possibly allowing for their combination to enhance the study of carcinogenesis and early cancer diagnostics in the future.

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