Anti-Hyperglycemic Effects of Thai Herbal Medicines

Overview

Journal Plants (Basel)

Date 2024 Oct 26

PMID 39458809

Authors

Athit Bunyakitcharoen

Weerakit Taychaworaditsakul

Seewaboon Sireeratawong

Sunee Chansakaow

Affiliations

Soon will be listed here.

Abstract

This study aims to investigate selected medicinal plants' anti-oxidative and antihyperglycemic activities to develop an effective remedy for lowering blood glucose levels and/or reducing diabetes complications. Thai medicinal plants, reported to have blood sugar-lowering effects, were selected for the study: , , , , , , and . Each species was extracted by Soxhlet's extraction using ethanol as solvent. The ethanolic crude extract of each species was then evaluated for its phytochemicals, anti-oxidant, and antihyperglycemic activities. The results showed that the extract of gave the highest values of total phenolic and total flavonoid content (167.95 mg gallic acid equivalents (GAE)/g and 81.70 mg CE/g, respectively). Anti-oxidant activity was determined using DPPH and ABTS radical scavenging activity. Among the ethanolic extracts, exhibited the highest anti-oxidant activity with IC values of 19.16 and 8.53 µg/mL, respectively. The antihyperglycemic activity was assessed using α-glucosidase inhibitory and glucose consumption activities. and demonstrated the highest α-glucosidase inhibitory activity among the ethanolic extracts, with IC values of 134.40 and 329.97 µg/mL, respectively. and showed the highest percentage of glucose consumption activity in induced insulin-resistant HepG2 cells at a concentration of 50 µg/mL with 145.16 and 107.03%, respectively. The results from α-glucosidase inhibitory and glucose consumption activities were developed as an effective antihyperglycemic remedy. Among the remedies tested, the R1 remedy exhibited the highest potential for reducing blood glucose levels, with an IC value of 122.10 µg/mL. Therefore, the R1 remedy should be further studied for its effects on animals.

Citing Articles

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References

Kao T, Huang S, Inbaraj B, Chen B . Determination of flavonoids and saponins in Gynostemma pentaphyllum (Thunb.) Makino by liquid chromatography-mass spectrometry. Anal Chim Acta. 2008; 626(2):200-11. DOI: 10.1016/j.aca.2008.07.049. View

Ahn E, Lee J, Jeon Y, Choi S, Kim E . Anti-diabetic effects of mulberry ( L.) branches and oxyresveratrol in streptozotocin-induced diabetic mice. Food Sci Biotechnol. 2018; 26(6):1693-1702. PMC: 6049727. DOI: 10.1007/s10068-017-0223-y. View

Pandey A, Tripathi P, Pandey R, Srivatava R, Goswami S . Alternative therapies useful in the management of diabetes: A systematic review. J Pharm Bioallied Sci. 2012; 3(4):504-12. PMC: 3249697. DOI: 10.4103/0975-7406.90103. View

Titchenell P, Lazar M, Birnbaum M . Unraveling the Regulation of Hepatic Metabolism by Insulin. Trends Endocrinol Metab. 2017; 28(7):497-505. PMC: 5477655. DOI: 10.1016/j.tem.2017.03.003. View

Nuchuchua O, Srinuanchai W, Chansriniyom C, Suttisansanee U, Temviriyanukul P, Nuengchamnong N . Relationship of phytochemicals and antioxidant activities in leaf extracts. Heliyon. 2024; 10(1):e23175. PMC: 10755283. DOI: 10.1016/j.heliyon.2023.e23175. View

Yang C, Yen Y, Hung K, Hsu S, Lan S, Lin H . Inhibitory effects of pu-erh tea on alpha glucosidase and alpha amylase: a systemic review. Nutr Diabetes. 2019; 9(1):23. PMC: 6712024. DOI: 10.1038/s41387-019-0092-y. View

Li H, Rafie R, Xu Z, Siddiqui R . Phytochemical profile and anti-oxidation activity changes during ginger () harvest: Baby ginger attenuates lipid accumulation and ameliorates glucose uptake in HepG2 cells. Food Sci Nutr. 2022; 10(1):133-144. PMC: 8751441. DOI: 10.1002/fsn3.2654. View

Bajaj S, Khan A . Antioxidants and diabetes. Indian J Endocrinol Metab. 2013; 16(Suppl 2):S267-71. PMC: 3603044. DOI: 10.4103/2230-8210.104057. View

Hossain U, Das A, Ghosh S, Sil P . An overview on the role of bioactive α-glucosidase inhibitors in ameliorating diabetic complications. Food Chem Toxicol. 2020; 145:111738. PMC: 7480666. DOI: 10.1016/j.fct.2020.111738. View

10.

Yang D, Chen X, Liu X, Han N, Liu Z, Li S . Antioxidant and -Glucosidase Inhibitory Activities Guided Isolation and Identification of Components from Mango Seed Kernel. Oxid Med Cell Longev. 2021; 2020:8858578. PMC: 7785352. DOI: 10.1155/2020/8858578. View

11.

Sadeghi Ekbatan S, Li X, Ghorbani M, Azadi B, Kubow S . Chlorogenic Acid and Its Microbial Metabolites Exert Anti-Proliferative Effects, S-Phase Cell-Cycle Arrest and Apoptosis in Human Colon Cancer Caco-2 Cells. Int J Mol Sci. 2018; 19(3). PMC: 5877584. DOI: 10.3390/ijms19030723. View

12.

Yee L, Mortimer J, Natarajan R, Dietze E, Seewaldt V . Metabolic Health, Insulin, and Breast Cancer: Why Oncologists Should Care About Insulin. Front Endocrinol (Lausanne). 2020; 11:58. PMC: 7045050. DOI: 10.3389/fendo.2020.00058. View

13.

Molinaro A, Becattini B, Solinas G . Insulin signaling and glucose metabolism in different hepatoma cell lines deviate from hepatocyte physiology toward a convergent aberrant phenotype. Sci Rep. 2020; 10(1):12031. PMC: 7374613. DOI: 10.1038/s41598-020-68721-9. View

14.

Thariwong S, Intharuksa A, Sirisa-Ard P, Charoensup W, Chansakaow S . Specification and DNA Barcoding of Thai Traditional Remedy for Chronic Kidney Disease: Pikad Tri-phol-sa-mut-than. Plants (Basel). 2021; 10(10). PMC: 8540904. DOI: 10.3390/plants10102023. View

15.

Xu B, Li Z, Zeng T, Zhan J, Wang S, Ho C . Bioactives of as Potential Anti-Diabetic/Hypoglycemic Agents. Molecules. 2022; 27(7). PMC: 9000558. DOI: 10.3390/molecules27072175. View

16.

Salehi B, Ata A, Anil Kumar N, Sharopov F, Ramirez-Alarcon K, Ruiz-Ortega A . Antidiabetic Potential of Medicinal Plants and Their Active Components. Biomolecules. 2019; 9(10). PMC: 6843349. DOI: 10.3390/biom9100551. View

17.

Chen Z, Du X, Yang Y, Cui X, Zhang Z, Li Y . Comparative study of chemical composition and active components against α-glucosidase of various medicinal parts of Morus alba L. Biomed Chromatogr. 2018; 32(11):e4328. DOI: 10.1002/bmc.4328. View

18.

Polumackanycz M, Wesolowski M, Viapiana A . Morus alba L. and Morus nigra L. Leaves as a Promising Food Source of Phenolic Compounds with Antioxidant Activity. Plant Foods Hum Nutr. 2021; 76(4):458-465. PMC: 8629867. DOI: 10.1007/s11130-021-00922-7. View

19.

Ibrahim I, Mohd Said M, Mohammad Zainoor N, Jamal J . Authentication of (Blume) Kuntze: A Systematic Review. Front Pharmacol. 2022; 13:855384. PMC: 9213798. DOI: 10.3389/fphar.2022.855384. View

20.

Chen C, Mohamad Razali U, Saikim F, Mahyudin A, Mohd Noor N . L. Plant: Bioactive Compounds and Potential as a Functional Food Ingredient. Foods. 2021; 10(3). PMC: 8004891. DOI: 10.3390/foods10030689. View