Three-Year Monitoring of Microorganisms' Composition and Concentration in Atmospheric Aerosols of Novosibirsk City and Suburbs

Overview

Journal Microorganisms

Specialty Microbiology

Date 2024 Oct 26

PMID 39458377

Authors

Irina Andreeva

Aleksandr Safatov

Olga Totmenina

Sergei Olkin

Maxim Rebus

Galina Buryak

Tatiana Alikina

Olga Baturina

Marsel Kabilov

Affiliations

Soon will be listed here.

Abstract

The atmospheric environment is formed under the influence of local and distant sources as a result of horizontal and vertical transport. In the present work, microbiological analysis of 604 samples of atmospheric aerosol collected in the period from September 2020 to September 2023 at four sites differing in anthropogenic load, located in Novosibirsk and the region, was carried out. Day and night aerosol samples were collected during 12 h every two weeks by filtration using Sartorius reinforced Teflon membranes, then sown on a set of nutrient media. The taxonomic affiliation of the isolated microbial isolates was determined based on phenotypic characteristics and analysis of 16S rRNA gene nucleotide sequences. Changes in the composition and concentration of culturable microorganisms depending on the season, time of day, and site of aerosol sampling were observed. In winter, lower fungi and bacteria of the genera , , dominated with an average concentration from zero to 12.5 CFU/m of aerosol. In the warm period, the concentration and diversity of cocci, spore-forming and non-spore-forming bacteria, actinomycetes, and fungi (up to 1970 CFU/m), among which pathogenic microorganisms were found, increased sharply in aerosols. The use of 16S metabarcoding techniques has greatly expanded the range of aerosols' microbial diversity detectable.

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