Chemical Profiling, Enzyme Inhibitory Activity and Antioxidant Capacity of South African Herbal Teas: , , and

Overview

Journal Antioxidants (Basel)

Date 2024 Oct 26

PMID 39456472

Authors

Nelida Nina

Alberto Burgos-Edwards

Cristina Theoduloz

Satyajit Tripathy

Motlalepula Gilbert Matsabisa

Guillermo Schmeda-Hirschmann

Affiliations

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Abstract

Herbal teas are used in South Africa as digestives to lower glycaemia and for other indications. However, the chemical composition of the infusions and their effect on enzymes related to metabolic syndrome is poorly known. The composition of infusions and methanol extracts of (Scrophulariaceae), , , and (Verbenaceae) was assessed and the effect of the infusions and extract was determined towards α-glucosidase, α-amylase, and pancreatic lipase. The commercial herbal products were extracted separately with MeOH or hot water to obtain the extract/infusion for comparative studies. Total phenolic, total flavonoid and antioxidant capacity were assessed. The fingerprints of the MeOH extracts and infusions were compared by HPLD-DAD. The extract constituents were tentatively identified by HPLC-MS/MS and NMR analyses. From the extracts/infusions, 57 compounds were identified, including iridoids, phenylpropanoid glycosides, flavonoids, and caffeic acid derivatives, among others. The MeOH extracts and infusions showed strong inhibition towards α-glucosidase with IC in the range of 0.13-0.84 µg/mL for the phenolic-enriched infusion extract (PEI) and 0.47-0.50 µg/mL for the MeOH extracts, respectively. The PEI showed higher inhibition towards α-glucosidase, and the MeOH extract of was better than the PEI. At 100 µg/mL, the PEI from the four herbal teas reduces the activity of α-amylase by 23.03-28.61%, with as the most active tea. Three of the species are high in phenylpropanoid glycosides, while contains rosmarinic acid. Some 26 compounds were identified in the infusion from , 28 from , and 21 from . Four of them are common in all the teas, namely decaffeoylverbascoside, verbascoside, isoverbascoside, and tuberonic acid hexoside. Ten compounds occur in two of the teas and seventeen, fifteen, and eleven compounds were detected only in , , and , respectively. Most of the compounds are reported for the first time from the crude drug infusions. The results give some support for the traditional use of herbal teas as digestives and/or indications for diabetes. The chemical fingerprints set the basis for quality control of the crude drugs, based on the main constituents and differential compounds occurring in the samples.

References

Ono M, Morinaga H, Masuoka C, Ikeda T, Okawa M, Kinjo J . New Bisabolane-Type Sesquiterpenes from the Aerial Parts of Lippia dulcis. Chem Pharm Bull (Tokyo). 2005; 53(9):1175-7. DOI: 10.1248/cpb.53.1175. View

Quirantes-Pine R, Funes L, Micol V, Segura-Carretero A, Fernandez-Gutierrez A . High-performance liquid chromatography with diode array detection coupled to electrospray time-of-flight and ion-trap tandem mass spectrometry to identify phenolic compounds from a lemon verbena extract. J Chromatogr A. 2009; 1216(28):5391-7. DOI: 10.1016/j.chroma.2009.05.038. View

Guo H, Liu A, Ye M, Yang M, Guo D . Characterization of phenolic compounds in the fruits of Forsythia suspensa by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry. Rapid Commun Mass Spectrom. 2007; 21(5):715-29. DOI: 10.1002/rcm.2875. View

Cao X, Qiao J, Wang L, Ye X, Zheng L, Jiang N . Screening of glycoside isomers in P. scrophulariiflora using ionic liquid-based ultrasonic-assisted extraction and ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry. Rapid Commun Mass Spectrom. 2012; 26(7):740-8. DOI: 10.1002/rcm.6158. View

Hvattum E, Ekeberg D . Study of the collision-induced radical cleavage of flavonoid glycosides using negative electrospray ionization tandem quadrupole mass spectrometry. J Mass Spectrom. 2003; 38(1):43-9. DOI: 10.1002/jms.398. View

Matsabisa M, Bala A, Tripathy S, Digashu M, Rautenbach F, Dassarma B . Study on South African Indigenous Teas-Antioxidant Potential, Nutritional Content, and Hypoxia-Induced Cyclooxygenase Inhibition on U87 MG Cell Line. Molecules. 2022; 27(11). PMC: 9181930. DOI: 10.3390/molecules27113505. View

Zhou T, Liu H, Wen J, Fan G, Chai Y, Wu Y . Fragmentation study of iridoid glycosides including epimers by liquid chromatography-diode array detection/electrospray ionization mass spectrometry and its application in metabolic fingerprint analysis of Gardenia jasminoides Ellis. Rapid Commun Mass Spectrom. 2010; 24(17):2520-8. DOI: 10.1002/rcm.4643. View

Lin L, Dong Y, Zhao H, Wen L, Yang B, Zhao M . Comparative evaluation of rosmarinic acid, methyl rosmarinate and pedalitin isolated from Rabdosia serra (MAXIM.) HARA as inhibitors of tyrosinase and α-glucosidase. Food Chem. 2014; 129(3):884-9. DOI: 10.1016/j.foodchem.2011.05.039. View

Ferreres F, Llorach R, Gil-Izquierdo A . Characterization of the interglycosidic linkage in di-, tri-, tetra- and pentaglycosylated flavonoids and differentiation of positional isomers by liquid chromatography/electrospray ionization tandem mass spectrometry. J Mass Spectrom. 2004; 39(3):312-21. DOI: 10.1002/jms.586. View

10.

Sun M, Luo Z, Liu Y, Yang R, Lu L, Yu G . Identification of the Major Components of Buddleja officinalis Extract and Their Metabolites in Rat Urine by UHPLC-LTQ-Orbitrap. J Food Sci. 2016; 81(10):H2587-H2596. DOI: 10.1111/1750-3841.13435. View

11.

Timoteo P, Karioti A, Leitao S, Vincieri F, Bilia A . A validated HPLC method for the analysis of herbal teas from three chemotypes of Brazilian Lippia alba. Food Chem. 2015; 175:366-73. DOI: 10.1016/j.foodchem.2014.11.129. View

12.

Tadera K, Minami Y, Takamatsu K, Matsuoka T . Inhibition of alpha-glucosidase and alpha-amylase by flavonoids. J Nutr Sci Vitaminol (Tokyo). 2006; 52(2):149-53. DOI: 10.3177/jnsv.52.149. View

13.

Muller C, Joubert E, de Beer D, Sanderson M, Malherbe C, Fey S . Acute assessment of an aspalathin-enriched green rooibos (Aspalathus linearis) extract with hypoglycemic potential. Phytomedicine. 2012; 20(1):32-9. DOI: 10.1016/j.phymed.2012.09.010. View

14.

Amoah S, Sandjo L, Kratz J, Biavatti M . Rosmarinic Acid--Pharmaceutical and Clinical Aspects. Planta Med. 2016; 82(5):388-406. DOI: 10.1055/s-0035-1568274. View

15.

De Rosso M, Tonidandel L, Larcher R, Nicolini G, Dalla Vedova A, De Marchi F . Identification of new flavonols in hybrid grapes by combined liquid chromatography-mass spectrometry approaches. Food Chem. 2014; 163:244-51. DOI: 10.1016/j.foodchem.2014.04.110. View

16.

Zhu F, Asada T, Sato A, Koi Y, Nishiwaki H, Tamura H . Rosmarinic acid extract for antioxidant, antiallergic, and α-glucosidase inhibitory activities, isolated by supramolecular technique and solvent extraction from Perilla leaves. J Agric Food Chem. 2014; 62(4):885-92. DOI: 10.1021/jf404318j. View

17.

Rastrelli L, Caceres A, Morales C, De Simone F, Aquino R . Iridoids from Lippia graveolens. Phytochemistry. 2001; 49(6):1829-1832. DOI: 10.1016/s0031-9422(98)00196-4. View

18.

Singh A, Venugopala K, Khedr M, Pillay M, Nwaeze K, Coovadia Y . Antimycobacterial, docking and molecular dynamic studies of pentacyclic triterpenes from Buddleja saligna leaves. J Biomol Struct Dyn. 2017; 35(12):2654-2664. DOI: 10.1080/07391102.2016.1227725. View

19.

Ferreres F, Gil-Izquierdo A, Andrade P, Valentao P, Tomas-Barberan F . Characterization of C-glycosyl flavones O-glycosylated by liquid chromatography-tandem mass spectrometry. J Chromatogr A. 2007; 1161(1-2):214-23. DOI: 10.1016/j.chroma.2007.05.103. View

20.

Funari C, Eugster P, Martel S, Carrupt P, Wolfender J, Silva D . High resolution ultra high pressure liquid chromatography-time-of-flight mass spectrometry dereplication strategy for the metabolite profiling of Brazilian Lippia species. J Chromatogr A. 2012; 1259:167-78. DOI: 10.1016/j.chroma.2012.03.069. View