Salinity As an Abiotic Stressor for Eliciting Bioactive Compounds in Marine Microalgae

Overview

Journal Toxins (Basel)

Publisher MDPI

Specialty Toxicology

Date 2024 Oct 25

PMID 39453201

Authors

Adrian Macias-de la Rosa

Lorenzo Lopez-Rosales

Antonio Contreras-Gomez

Asterio Sanchez-Miron

Francisco Garcia-Camacho

Maria Del Carmen Ceron-Garcia

Affiliations

Soon will be listed here.

Abstract

This study investigated the impact of culture medium salinity (5-50 PSU) on the growth and maximum photochemical yield of photosystem II (/) and the composition of carotenoids, fatty acids, and bioactive substances in three marine microalgae (, , and ). The microalgae were photoautotrophically cultured in discontinuous mode in a single stage (S1) and a two-stage culture with salt shock (S2). A growth model was developed to link biomass productivity with salinity for each species. achieved a maximum biomass productivity () of 15.85 ± 0.32 mg·L·day in S1 and 16.12 ± 0.13 mg·L·day in S2. The salt shock in S2 notably enhanced carotenoid production, particularly in and , where fucoxanthin was the main carotenoid, while peridinin dominated in . also exhibited increased fatty acid productivity in S2. Salinity changes affected the proportions of saturated, monounsaturated, and polyunsaturated fatty acids in all three species. Additionally, hyposaline conditions boosted the production of haemolytic substances in and

Citing Articles

Extraction and Analytical Methods for the Characterization of Polyphenols in Marine Microalgae: A Review.

Bermudez G, Terenzi C, Medri F, Andrisano V, Montanari S Mar Drugs. 2024; 22(12).

PMID: 39728113 PMC: 11678617. DOI: 10.3390/md22120538.

References

Xia L, Ge H, Zhou X, Zhang D, Hu C . Photoautotrophic outdoor two-stage cultivation for oleaginous microalgae Scenedesmus obtusus XJ-15. Bioresour Technol. 2013; 144:261-7. DOI: 10.1016/j.biortech.2013.06.112. View

Ross T . Indices for performance evaluation of predictive models in food microbiology. J Appl Bacteriol. 1996; 81(5):501-8. DOI: 10.1111/j.1365-2672.1996.tb03539.x. View

Lim P, Ogata T . Salinity effect on growth and toxin production of four tropical Alexandrium species (Dinophyceae). Toxicon. 2005; 45(6):699-710. DOI: 10.1016/j.toxicon.2005.01.007. View

Guillard R, Ryther J . Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea (cleve) Gran. Can J Microbiol. 1962; 8:229-39. DOI: 10.1139/m62-029. View

Flores-Lenero A, Vargas-Torres V, Paredes-Mella J, Norambuena L, Fuenzalida G, Lee-Chang K . in Patagonian Fjords: Genetics, Growth, Pigment Signature and Role of PUFA and ROS in Ichthyotoxicity. Toxins (Basel). 2022; 14(9). PMC: 9504362. DOI: 10.3390/toxins14090577. View

Fal S, Aasfar A, Rabie R, Smouni A, El Arroussi H . Salt induced oxidative stress alters physiological, biochemical and metabolomic responses of green microalga . Heliyon. 2022; 8(1):e08811. PMC: 8792077. DOI: 10.1016/j.heliyon.2022.e08811. View

Assuncao J, Guedes A, Malcata F . Biotechnological and Pharmacological Applications of Biotoxins and Other Bioactive Molecules from Dinoflagellates. Mar Drugs. 2017; 15(12). PMC: 5742853. DOI: 10.3390/md15120393. View

Ren Y, Sun H, Deng J, Huang J, Chen F . Carotenoid Production from Microalgae: Biosynthesis, Salinity Responses and Novel Biotechnologies. Mar Drugs. 2021; 19(12). PMC: 8708220. DOI: 10.3390/md19120713. View

Abreu A, Molina-Miras A, Aguilera-Saez L, Lopez-Rosales L, Ceron-Garcia M, Sanchez-Miron A . Production of Amphidinols and Other Bioproducts of Interest by the Marine Microalga Unraveled by Nuclear Magnetic Resonance Metabolomics Approach Coupled to Multivariate Data Analysis. J Agric Food Chem. 2019; 67(34):9667-9682. DOI: 10.1021/acs.jafc.9b02821. View

10.

Macias-de la Rosa A, Gonzalez-Cardoso M, Ceron-Garcia M, Lopez-Rosales L, Gallardo-Rodriguez J, Seoane S . Bioactives Overproduction through Operational Strategies in the Ichthyotoxic Microalga Culture. Toxins (Basel). 2023; 15(5). PMC: 10224309. DOI: 10.3390/toxins15050349. View

11.

Thi Nhu Bui Q, Kim H, Park H, Ki J . Salinity Affects Saxitoxins (STXs) Toxicity in the Dinoflagellate , with Low Transcription of SXT-Biosynthesis Genes and . Toxins (Basel). 2021; 13(10). PMC: 8539879. DOI: 10.3390/toxins13100733. View

12.

Smaoui S, Barkallah M, Ben Hlima H, Fendri I, Khaneghah A, Michaud P . Microalgae Xanthophylls: From Biosynthesis Pathway and Production Techniques to Encapsulation Development. Foods. 2021; 10(11). PMC: 8623138. DOI: 10.3390/foods10112835. View

13.

Chen H, Xue L, Liang M, Jiang J . Sodium azide intervention, salinity stress and two-step cultivation of Dunaliella tertiolecta for lipid accumulation. Enzyme Microb Technol. 2019; 127:1-5. DOI: 10.1016/j.enzmictec.2019.04.008. View

14.

Paliwal C, Mitra M, Bhayani K, Vamsi Bharadwaj S, Ghosh T, Dubey S . Abiotic stresses as tools for metabolites in microalgae. Bioresour Technol. 2017; 244(Pt 2):1216-1226. DOI: 10.1016/j.biortech.2017.05.058. View

15.

Molina-Miras A, Lopez-Rosales L, Sanchez-Miron A, Ceron-Garcia M, Seoane-Parra S, Garcia-Camacho F . Long-term culture of the marine dinoflagellate microalga Amphidinium carterae in an indoor LED-lighted raceway photobioreactor: Production of carotenoids and fatty acids. Bioresour Technol. 2018; 265:257-267. DOI: 10.1016/j.biortech.2018.05.104. View

16.

Chisti Y . Biodiesel from microalgae. Biotechnol Adv. 2007; 25(3):294-306. DOI: 10.1016/j.biotechadv.2007.02.001. View

17.

Wang H, Postier B, Burnap R . Polymerase chain reaction-based mutageneses identify key transporters belonging to multigene families involved in Na+ and pH homeostasis of Synechocystis sp. PCC 6803. Mol Microbiol. 2002; 44(6):1493-506. DOI: 10.1046/j.1365-2958.2002.02983.x. View

18.

Lopez-Rosales L, Gallardo-Rodriguez J, Sanchez-Miron A, Ceron-Garcia M, Belarbi E, Garcia-Camacho F . Simultaneous effect of temperature and irradiance on growth and okadaic acid production from the marine dinoflagellate Prorocentrum belizeanum. Toxins (Basel). 2014; 6(1):229-53. PMC: 3920259. DOI: 10.3390/toxins6010229. View

19.

Lafarga T, Acien G . Microalgae for the Food Industry: From Biomass Production to the Development of Functional Foods. Foods. 2022; 11(5). PMC: 8908970. DOI: 10.3390/foods11050765. View

20.

Macias-de la Rosa A, Lopez-Rosales L, Ceron-Garcia M, Molina-Miras A, Soriano-Jerez Y, Sanchez-Miron A . Assessment of the marine microalga Chrysochromulina rotalis as bioactive feedstock cultured in an easy-to-deploy light-emitting-diode-based tubular photobioreactor. Bioresour Technol. 2023; 389:129818. DOI: 10.1016/j.biortech.2023.129818. View