Regarding Bioanalysis Lasting a Few Minutes: Automated Cooling-SPME and Fast-GC for Urinary 2-Phenyl-2-Propanol Monitoring

Overview

Journal Toxics

Date 2024 Oct 25

PMID 39453163

Authors

Stefano Dugheri

Niccolo Fanfani

Giovanni Cappelli

Antonio Marigliano

Elisabetta Bucaletti

Donato Squillaci

Ilaria Rapi

Lorenzo Venturini

Giulia Pizzella

Sara Manetta

Alfonso Pavone

Michele Secchi

Iacopo Rainaldi

Nicola Mucci

Affiliations

Soon will be listed here.

Abstract

An innovative SPME head space GC-MS method, in cooling mode, using a fully automated routine, was developed to detect 2-phenyl-2-propanol, a representative urinary metabolite of cumene. Following an acid hydrolysis and derivatization step with lowered quantities of reagents, acetic anhydride and pyridine, a 30 μm polydimethylsiloxane SPME fiber was used to sample derivatized 2-phenyl-2-propanol, such as benzenemethanol,α,α-dimethyl-acetate, from the headspace. Performances of the method, optimized through experimental design, provide an LOD of 0.034 mg/L and an LOQ 0.10 mg/L, with a short sampling time necessary per sample. The method, developed on standard solutions, will be applied to both occupationally exposed and non-exposed populations.

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