Unveiling the Potential of CuO and CuO Nanoparticles Against Novel Copper-Resistant Strains: An In-Depth Comparison

Overview

Journal Nanomaterials (Basel)

Date 2024 Oct 25

PMID 39452980

Authors

Olesia Havryliuk

Garima Rathee

Jeniffer Blair

Vira Hovorukha

Oleksandr Tashyrev

Jordi Morato

Leonardo M Perez

Tzanko Tzanov

Affiliations

Soon will be listed here.

Abstract

Four novel strains with record resistance to copper (Cu) previously isolated from ecologically diverse samples ( UKR1, UKR2, UKR3, and UKR4) were tested against sonochemically synthesised copper-oxide (I) (CuO) and copper-oxide (II) (CuO) nanoparticles (NPs). Nanomaterials characterisation by X-ray diffractometry (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), and High-Resolution Transmission Electron Microscopy (HRTEM) confirmed the synthesis of CuO and CuO NPs. CuO NPs exhibited better performance in inhibiting bacterial growth due to their heightened capacity to induce oxidative stress. The greater stability and geometrical shape of CuO NPs were disclosed as important features associated with bacterial cell toxicity. SEM and TEM images confirmed that both NPs caused membrane disruption, altered cell morphology, and pronounced membrane vesiculation, a distinctive feature of bacteria dealing with stressor factors. Finally, CuO and CuO NPs effectively decreased the biofilm-forming ability of the Cu-resistant UKR strains as well as degraded pre-established biofilm, matching NPs' antimicrobial performance. Despite the similarities in the mechanisms of action revealed by both NPs, distinctive behaviours were also detected for the different species of wild-type analysed. In summary, these findings underscore the efficacy of nanotechnology-driven strategies for combating metal tolerance in bacteria.

References

Bogdanovic U, Vodnik V, Mitric M, Dimitrijevic S, Skapin S, Zunic V . Nanomaterial with high antimicrobial efficacy--copper/polyaniline nanocomposite. ACS Appl Mater Interfaces. 2015; 7(3):1955-66. DOI: 10.1021/am507746m. View

Nurzhanova F, Absatirov G, Sidikhov B, Sidorchuk A, Ginayatov N, Murzabaev K . The vulnerary potential of botanical medicines in the treatment of bacterial pathologies in fish. Vet World. 2021; 14(3):551-557. PMC: 8076460. DOI: 10.14202/vetworld.2021.551-557. View

Slavin Y, Ivanova K, Hoyo J, Perelshtein I, Owen G, Haegert A . Novel Lignin-Capped Silver Nanoparticles against Multidrug-Resistant Bacteria. ACS Appl Mater Interfaces. 2021; 13(19):22098-22109. DOI: 10.1021/acsami.0c16921. View

Punniyakotti P, Panneerselvam P, Perumal D, Aruliah R, Angaiah S . Anti-bacterial and anti-biofilm properties of green synthesized copper nanoparticles from Cardiospermum halicacabum leaf extract. Bioprocess Biosyst Eng. 2020; 43(9):1649-1657. DOI: 10.1007/s00449-020-02357-x. View

Wozniak-Budych M, Przysiecka L, Maciejewska B, Wieczorek D, Staszak K, Jarek M . Facile Synthesis of Sulfobetaine-Stabilized CuO Nanoparticles and Their Biomedical Potential. ACS Biomater Sci Eng. 2021; 3(12):3183-3194. DOI: 10.1021/acsbiomaterials.7b00465. View

Ghasemian E, Naghoni A, Rahvar H, Kialha M, Tabaraie B . Evaluating the Effect of Copper Nanoparticles in Inhibiting Pseudomonas aeruginosa and Listeria monocytogenes Biofilm Formation. Jundishapur J Microbiol. 2015; 8(5):e17430. PMC: 4537522. DOI: 10.5812/jjm.8(5)2015.17430. View

Xu X, Brownlow W, Kyriacou S, Wan Q, Viola J . Real-time probing of membrane transport in living microbial cells using single nanoparticle optics and living cell imaging. Biochemistry. 2004; 43(32):10400-13. DOI: 10.1021/bi036231a. View

Sundar S, Venkatachalam G, Kwon S . Biosynthesis of Copper Oxide (CuO) Nanowires and Their Use for the Electrochemical Sensing of Dopamine. Nanomaterials (Basel). 2018; 8(10). PMC: 6215139. DOI: 10.3390/nano8100823. View

Ethiraj A, Joon Kang D . Synthesis and characterization of CuO nanowires by a simple wet chemical method. Nanoscale Res Lett. 2012; 7(1):70. PMC: 3283496. DOI: 10.1186/1556-276X-7-70. View

10.

Priya M, Venkatesan R, Deepa S, Sana S, Arumugam S, Karami A . Green synthesis, characterization, antibacterial, and antifungal activity of copper oxide nanoparticles derived from Morinda citrifolia leaf extract. Sci Rep. 2023; 13(1):18838. PMC: 10620180. DOI: 10.1038/s41598-023-46002-5. View

11.

Draviana H, Fitriannisa I, Khafid M, Krisnawati D, Widodo , Lai C . Size and charge effects of metal nanoclusters on antibacterial mechanisms. J Nanobiotechnology. 2023; 21(1):428. PMC: 10648733. DOI: 10.1186/s12951-023-02208-3. View

12.

Ferreres G, Ivanova K, Torrent-Burgues J, Tzanov T . Multimodal silver-chitosan-acylase nanoparticles inhibit bacterial growth and biofilm formation by Gram-negative Pseudomonas aeruginosa bacterium. J Colloid Interface Sci. 2023; 646:576-586. DOI: 10.1016/j.jcis.2023.04.184. View

13.

Havryliuk O, Hovorukha V, Patrauchan M, Youssef N, Tashyrev O . Draft whole genome sequence for four highly copper resistant soil isolates s strain UKR1, strain UKR2, and strains UKR3 and UKR4. Curr Res Microb Sci. 2021; 1:44-52. PMC: 8610347. DOI: 10.1016/j.crmicr.2020.06.002. View

14.

Pal S, Tak Y, Song J . Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the Gram-negative bacterium Escherichia coli. Appl Environ Microbiol. 2007; 73(6):1712-20. PMC: 1828795. DOI: 10.1128/AEM.02218-06. View

15.

von Neubeck M, Huptas C, Gluck C, Krewinkel M, Stoeckel M, Stressler T . Pseudomonas lactis sp. nov. and Pseudomonas paralactis sp. nov., isolated from bovine raw milk. Int J Syst Evol Microbiol. 2017; 67(6):1656-1664. DOI: 10.1099/ijsem.0.001836. View

16.

Kaur R, Kaur K, Alyami M, Kaur Lang D, Saini B, Bayan M . Combating Microbial Infections Using Metal-Based Nanoparticles as Potential Therapeutic Alternatives. Antibiotics (Basel). 2023; 12(5). PMC: 10215692. DOI: 10.3390/antibiotics12050909. View

17.

Muhammad M, Idris A, Fan X, Guo Y, Yu Y, Jin X . Beyond Risk: Bacterial Biofilms and Their Regulating Approaches. Front Microbiol. 2020; 11:928. PMC: 7253578. DOI: 10.3389/fmicb.2020.00928. View

18.

Bezza F, Tichapondwa S, Chirwa E . Fabrication of monodispersed copper oxide nanoparticles with potential application as antimicrobial agents. Sci Rep. 2020; 10(1):16680. PMC: 7541485. DOI: 10.1038/s41598-020-73497-z. View

19.

Ortiz de Orue Lucana D, Wedderhoff I, Groves M . ROS-Mediated Signalling in Bacteria: Zinc-Containing Cys-X-X-Cys Redox Centres and Iron-Based Oxidative Stress. J Signal Transduct. 2011; 2012:605905. PMC: 3184428. DOI: 10.1155/2012/605905. View

20.

Sahli C, Moya S, Lomas J, Gravier-Pelletier C, Briandet R, Hemadi M . Recent advances in nanotechnology for eradicating bacterial biofilm. Theranostics. 2022; 12(5):2383-2405. PMC: 8899562. DOI: 10.7150/thno.67296. View