Integrated Hormone and Transcriptome Profiles Provide Insight into the Pericarp Differential Development Mechanism Between Mandarin 'Shatangju' and 'Chunhongtangju'

Overview

Journal Front Plant Sci

Date 2024 Oct 25

PMID 39450074

Authors

Yongjing Huang

Congyi Zhu

Yibo Hu

Sanjiao Yan

Zhimin Luo

Yanping Zou

Wen Wu

Jiwu Zeng

Affiliations

Soon will be listed here.

Abstract

Introduction: cv. 'Chunhongtangju' was mutated from Mandarin 'Shatangju', which has been identified as a new citrus variety. Mandarin 'Chunhongtangju' fruits were late-ripening for about two months than Mandarin 'Shatangju'.

Methods: To understand the pericarp differential development mechanism in Mandarin 'Shatangju' (CK) and 'Chunhongtangju' (LM), hormones and transcriptome profiles of pericarps were performed in different development stages: Young fruit stage (CK1/LM1), Expansion and Turning color stage (CK2), Expansion stage (LM2), Turning color stage (LM3), and Maturity stage (CK3/LM4).

Results: In this study, the development of LM was significantly slower, and the maturity was significantly delayed. At the same stage, most hormones in Mandarin 'Chunhongtangju' pericarps were higher than that in 'Shatangju' such as gibberellin A24, cis(+)-12-oxophytodienoic acid, and L-phenylalanine. The deficiency of hormones in late-maturing pericarps was mainly manifested in ABA, 12-OHJA, MeSAG, and ABA-GE. Differences in transcriptome profiles between the two citrus varieties are primarily observed in energy metabolism, signal transduction such as MAPK signaling pathway and plant hormone signaling, and biosynthesis of secondary metabolites. After analyzing the hormones and transcriptome data, we found that the top genes and hormones, such as Cs_ont_5g020040 (transcription elongation factor, ), Cs_ont_7g021670 (BAG family molecular chaperone regulator 5, ), Cs_ont_2g025760 (40S ribosomal protein S27, ), 5-deoxystrigol, salicylic acid 2-O-β-glucosid, and gibberellin A24, contributed significantly to gene transcription and hormone synthesis.

Discussion: This study suggests that the variances of pericarp development between the two varieties are linked to variations in the transcription levels of genes associated with energy and secondary metabolism, signal transduction related genes. These findings expand our understanding of the complex transcriptional and hormonal regulatory hierarchy during pericarp development.

References

Theissen G, Melzer R . Molecular mechanisms underlying origin and diversification of the angiosperm flower. Ann Bot. 2007; 100(3):603-19. PMC: 2533597. DOI: 10.1093/aob/mcm143. View

Chen J, Shi Y, Zhong Y, Sun Z, Niu J, Wang Y . Transcriptome Analysis and HPLC Profiling of Flavonoid Biosynthesis in L. during Its Key Developmental Stages. Biology (Basel). 2022; 11(7). PMC: 9313048. DOI: 10.3390/biology11071078. View

Yu X, Zhang Y, Wang D, Jiang L, Xu X . Identification of Three Kinds of Citri Reticulatae Pericarpium Based on Deoxyribonucleic Acid Barcoding and High-performance Liquid Chromatography-diode Array Detection-electrospray Ionization/Mass Spectrometry/Mass Spectrometry Combined with.... Pharmacogn Mag. 2018; 14(53):64-69. PMC: 5858244. DOI: 10.4103/pm.pm_581_16. View

Wang F, Wu Y, Wu W, Huang Y, Zhu C, Zhang R . Integrative analysis of metabolome and transcriptome profiles provides insight into the fruit pericarp pigmentation disorder caused by 'Candidatus Liberibacter asiaticus' infection. BMC Plant Biol. 2021; 21(1):397. PMC: 8385863. DOI: 10.1186/s12870-021-03167-3. View

Park M, Malka S . Gibberellin delays metabolic shift during tomato ripening by inducing auxin signaling. Front Plant Sci. 2022; 13:1045761. PMC: 9703062. DOI: 10.3389/fpls.2022.1045761. View

Shan W, Kuang J, Chen L, Xie H, Peng H, Xiao Y . Molecular characterization of banana NAC transcription factors and their interactions with ethylene signalling component EIL during fruit ripening. J Exp Bot. 2012; 63(14):5171-87. PMC: 3430993. DOI: 10.1093/jxb/ers178. View

Giovannoni J . Genetic regulation of fruit development and ripening. Plant Cell. 2004; 16 Suppl:S170-80. PMC: 2643394. DOI: 10.1105/tpc.019158. View

Bi X, Liao L, Deng L, Jin Z, Huang Z, Sun G . Combined Transcriptome and Metabolome Analyses Reveal Candidate Genes Involved in Tangor () Fruit Development and Quality Formation. Int J Mol Sci. 2022; 23(10). PMC: 9141862. DOI: 10.3390/ijms23105457. View

Lin Q, Wang C, Dong W, Jiang Q, Wang D, Li S . Transcriptome and metabolome analyses of sugar and organic acid metabolism in Ponkan (Citrus reticulata) fruit during fruit maturation. Gene. 2014; 554(1):64-74. DOI: 10.1016/j.gene.2014.10.025. View

10.

Wang J, Liu J, Chen K, Li H, He J, Guan B . Comparative transcriptome and proteome profiling of two Citrus sinensis cultivars during fruit development and ripening. BMC Genomics. 2017; 18(1):984. PMC: 5740884. DOI: 10.1186/s12864-017-4366-2. View

11.

Shumskaya M, Wurtzel E . The carotenoid biosynthetic pathway: thinking in all dimensions. Plant Sci. 2013; 208:58-63. PMC: 3672397. DOI: 10.1016/j.plantsci.2013.03.012. View

12.

Osorio S, Scossa F, Fernie A . Molecular regulation of fruit ripening. Front Plant Sci. 2013; 4:198. PMC: 3682129. DOI: 10.3389/fpls.2013.00198. View

13.

Zhang J, Zhang Q, Zhang H, Ma Q, Lu J, Qiao Y . Characterization of polymethoxylated flavonoids (PMFs) in the peels of 'Shatangju' mandarin ( Citrus reticulata Blanco) by online high-performance liquid chromatography coupled to photodiode array detection and electrospray tandem mass spectrometry. J Agric Food Chem. 2012; 60(36):9023-34. DOI: 10.1021/jf302713c. View

14.

Fujisawa M, Nakano T, Ito Y . Identification of potential target genes for the tomato fruit-ripening regulator RIN by chromatin immunoprecipitation. BMC Plant Biol. 2011; 11:26. PMC: 3039564. DOI: 10.1186/1471-2229-11-26. View

15.

Salehin M, Li B, Tang M, Katz E, Song L, Ecker J . Auxin-sensitive Aux/IAA proteins mediate drought tolerance in Arabidopsis by regulating glucosinolate levels. Nat Commun. 2019; 10(1):4021. PMC: 6731224. DOI: 10.1038/s41467-019-12002-1. View

16.

Gapper N, McQuinn R, Giovannoni J . Molecular and genetic regulation of fruit ripening. Plant Mol Biol. 2013; 82(6):575-91. DOI: 10.1007/s11103-013-0050-3. View

17.

Ku A, Huang Y, Wang Y, Ma D, Yeh K . IbMADS1 (Ipomoea batatas MADS-box 1 gene) is involved in tuberous root initiation in sweet potato (Ipomoea batatas). Ann Bot. 2008; 102(1):57-67. PMC: 2712425. DOI: 10.1093/aob/mcn067. View

18.

Azoulay Shemer T, Harpaz-Saad S, Belausov E, Lovat N, Krokhin O, Spicer V . Citrus chlorophyllase dynamics at ethylene-induced fruit color-break: a study of chlorophyllase expression, posttranslational processing kinetics, and in situ intracellular localization. Plant Physiol. 2008; 148(1):108-18. PMC: 2528117. DOI: 10.1104/pp.108.124933. View

19.

Wang L, Wang S, Li W . RSeQC: quality control of RNA-seq experiments. Bioinformatics. 2012; 28(16):2184-5. DOI: 10.1093/bioinformatics/bts356. View

20.

Sakuraba Y, Kim Y, Yoo S, Hortensteiner S, Paek N . 7-Hydroxymethyl chlorophyll a reductase functions in metabolic channeling of chlorophyll breakdown intermediates during leaf senescence. Biochem Biophys Res Commun. 2012; 430(1):32-7. DOI: 10.1016/j.bbrc.2012.11.050. View