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ISeq: an Integrated Tool to Fetch Public Sequencing Data

Overview
Journal Bioinformatics
Publisher Oxford University Press
Specialty Biology
Date 2024 Oct 24
PMID 39447029
Authors
Haoyu Chao
Zhuojin Li
Dijun Chen
Ming Chen
Affiliations
Soon will be listed here.
Abstract

Motivation: High-throughput sequencing technologies [next-generation sequencing (NGS)] are increasingly used to address diverse biological questions. Despite the rich information in NGS data, particularly with the growing datasets from repositories like the Genome Sequence Archive (GSA) at NGDC, programmatic access to public sequencing data and metadata remains limited.

Results: We developed iSeq to enable quick and straightforward retrieval of metadata and NGS data from multiple databases via the command-line interface. iSeq supports simultaneous retrieval from GSA, SRA, ENA, and DDBJ databases. It handles over 25 different accession formats, supports Aspera downloads, parallel downloads, multi-threaded processes, FASTQ file merging, and integrity verification, simplifying data acquisition and enhancing the capacity for reanalyzing NGS data.

Availability And Implementation: iSeq is freely available on Bioconda (https://anaconda.org/bioconda/iseq) and GitHub (https://github.com/BioOmics/iSeq).

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