The Role and Function of Secretory Protein Matrix Metalloproteinase-3 () in Cervical Cancer

Overview

Journal Iran J Public Health

Date 2024 Oct 24

PMID 39444485

Authors

Lei Shao

Wanqiu Liu

Chunyan Zhang

Wei Ma

Xiao Yu

Jing Han

Xiaojuan Wang

Affiliations

Soon will be listed here.

Abstract

Background: We started with RNA-seq analysis and aimed to investigate the possibility of secretory protein matrix metalloproteinase-3() as a new diagnosis and therapeutic target in cervical cancer.

Methods: The study was conducted on Nov 2021 at the Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, China. Through conjoint analysis of gene expression data as well as survival rate data, we explored the potential secretary proteins associated with cervical cancer carcinogenesis. One hundred patients aged 38-72 years with clinical stage I-IV cervical cancer, and 100 age-matched healthy women were included. The expression changes in serum of clinical patients was detected. We knockdown or overexpressed the secretory proteins then explored its influence on biological function of cervical cancer cells.

Results: By cross-analysis of The Cancer Genome Atlas (TCGA) database and MetazSecKB database, gene was most significantly upregulated in cervical cancer patients ( < 0.05). Furthermore, protein was remarkably increased in the serum of clinical cervical cancer patients and decreased after receiving treatment. Overexpression of in HT-3 cells or culturing new cells using the supernatant of the medium after overexpression could increase cell viability ( < 0.05) as well as proliferation ( < 0.05). Knockdown of reduced the phosphorylation of PI3K as well as AKT proteins, while the PI3K phosphorylation inhibitors could suppress the impact of on increasing cell proliferation as well as viability.

Conclusion: could be an underlying target for early diagnosis and treat cervical cancer in the future.

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