LncRNA MSTRG.13,871/miR155-5p/Grip1 Network Involved in the Post-cardiac Arrest Brain Injury

Overview

Journal Sci Rep

Specialty Science

Date 2024 Oct 24

PMID 39443577

Authors

Yiwei Li

Chenghao Wu

Xin Wen

Wei Hu

Mengyuan Diao

Affiliations

Soon will be listed here.

Abstract

Post-cardiac arrest brain (PCABI) is a severe medical condition characterized by a significant risk of neurological impairment and death. Nevertheless, the specific process and essential molecules responsible for its development are not fully understood. Profiling based on competing endogenous RNAs (ceRNA) has been implicated in the onset and progression of neurological disorders, yet its role in PCABI remains unclear. In this study, we performed RNA transcriptome sequencing analysis to identify differentially expressed genes in the rat model for cardiac arrest and cardiopulmonary resuscitation (CA/CPR). A hub ceRNA regulatory network was constructed using miRWalk 2.0 and Cytohubba plug-in in Cytoscape. Subsequently, real-time quantitative reverse transcription-polymerase chain reaction and dual-luciferase activity assays validated MSTRG.13,871, miR-155-5p, and Grip1 as differentially expressed in CA/CPR group, with MSTRG.13,871 capable of targeting both miR-155-5p and Grip1. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed the ceRNA network enrichment in immunoregulation mechanisms such as mitochondrion, apoptotic process, and negative regulation cell death. Our research highlights the mechanism of PCABI by revealing a critical regulatory axis involving MSTRG.13,871-miR-155-5p-Grip1 in the hippocampus CA1 region after CA/CPR in rats, proposing a feasible controlled mechanism, which may serve as a theoretical basis for designing innovative therapies.

References

Yu S, Wu C, Zhu Y, Diao M, Hu W . Rat model of asphyxia-induced cardiac arrest and resuscitation. Front Neurosci. 2023; 16:1087725. PMC: 9846144. DOI: 10.3389/fnins.2022.1087725. View

Wyszynski M, Kim E, Dunah A, Passafaro M, Valtschanoff J, Serra-Pages C . Interaction between GRIP and liprin-alpha/SYD2 is required for AMPA receptor targeting. Neuron. 2002; 34(1):39-52. DOI: 10.1016/s0896-6273(02)00640-2. View

Chatterjee P, Roy D . Structural insight into GRIP1-PDZ6 in Alzheimer's disease: study from protein expression data to molecular dynamics simulations. J Biomol Struct Dyn. 2016; 35(10):2235-2247. DOI: 10.1080/07391102.2016.1214085. View

Huang L, Zou J, Lu Q . Silencing rno-miR-155-5p in rat temporal lobe epilepsy model reduces pathophysiological features and cell apoptosis by activating Sestrin-3. Brain Res. 2017; 1689:109-122. DOI: 10.1016/j.brainres.2017.11.019. View

Zhang M, Liu Q, Meng H, Duan H, Liu X, Wu J . Ischemia-reperfusion injury: molecular mechanisms and therapeutic targets. Signal Transduct Target Ther. 2024; 9(1):12. PMC: 10772178. DOI: 10.1038/s41392-023-01688-x. View

Wang W, Shi J, Chen M, Zhuge X, Dai C, Xie L . Calpain inhibitor MDL28170 alleviates cerebral ischemia‑reperfusion injury by suppressing inflammation and autophagy in a rat model of cardiac arrest. Exp Ther Med. 2023; 25(5):196. PMC: 10119620. DOI: 10.3892/etm.2023.11895. View

Pertea M, Pertea G, Antonescu C, Chang T, Mendell J, Salzberg S . StringTie enables improved reconstruction of a transcriptome from RNA-seq reads. Nat Biotechnol. 2015; 33(3):290-5. PMC: 4643835. DOI: 10.1038/nbt.3122. View

Yuan L, Chen W, Xiang J, Deng Q, Hu Y, Li J . Advances of circRNA-miRNA-mRNA regulatory network in cerebral ischemia/reperfusion injury. Exp Cell Res. 2022; 419(2):113302. DOI: 10.1016/j.yexcr.2022.113302. View

Ruan H, Li Y, Wang C, Jiang Y, Han Y, Li Y . Click chemistry extracellular vesicle/peptide/chemokine nanocarriers for treating central nervous system injuries. Acta Pharm Sin B. 2023; 13(5):2202-2218. PMC: 10213615. DOI: 10.1016/j.apsb.2022.06.007. View

10.

Sun L, Luo H, Bu D, Zhao G, Yu K, Zhang C . Utilizing sequence intrinsic composition to classify protein-coding and long non-coding transcripts. Nucleic Acids Res. 2013; 41(17):e166. PMC: 3783192. DOI: 10.1093/nar/gkt646. View

11.

Yu W, Charych E, Serwanski D, Li R, Ali R, Bahr B . Gephyrin interacts with the glutamate receptor interacting protein 1 isoforms at GABAergic synapses. J Neurochem. 2008; 105(6):2300-14. PMC: 2891215. DOI: 10.1111/j.1471-4159.2008.05311.x. View

12.

Chen W, Wang L, Liu Z . MicroRNA-155 influences cell damage in ischemic stroke via TLR4/MYD88 signaling pathway. Bioengineered. 2021; 12(1):2449-2458. PMC: 8806472. DOI: 10.1080/21655979.2021.1935066. View

13.

Yang K, Zeng L, Ge A, Wang S, Zeng J, Yuan X . A systematic review of the research progress of non-coding RNA in neuroinflammation and immune regulation in cerebral infarction/ischemia-reperfusion injury. Front Immunol. 2022; 13:930171. PMC: 9585453. DOI: 10.3389/fimmu.2022.930171. View

14.

Nakanishi S . Molecular diversity of glutamate receptors and implications for brain function. Science. 1992; 258(5082):597-603. DOI: 10.1126/science.1329206. View

15.

Braithwaite S, Xia H, Malenka R . Differential roles for NSF and GRIP/ABP in AMPA receptor cycling. Proc Natl Acad Sci U S A. 2002; 99(10):7096-101. PMC: 124534. DOI: 10.1073/pnas.102156099. View

16.

Sekhon M, Ainslie P, Griesdale D . Clinical pathophysiology of hypoxic ischemic brain injury after cardiac arrest: a "two-hit" model. Crit Care. 2017; 21(1):90. PMC: 5390465. DOI: 10.1186/s13054-017-1670-9. View

17.

Nesseler N, Leurent G, Seguin P . Neurologic prognosis after cardiac arrest. N Engl J Med. 2009; 361(20):1999. DOI: 10.1056/NEJMc091781. View

18.

Smaili S, Hsu Y, Youle R, Russell J . Mitochondria in Ca2+ signaling and apoptosis. J Bioenerg Biomembr. 2002; 32(1):35-46. DOI: 10.1023/a:1005508311495. View

19.

Trapnell C, Williams B, Pertea G, Mortazavi A, Kwan G, van Baren M . Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol. 2010; 28(5):511-5. PMC: 3146043. DOI: 10.1038/nbt.1621. View

20.

Wu X, Wei H, Wu J . Coding and long non-coding gene expression changes in the CNS traumatic injuries. Cell Mol Life Sci. 2022; 79(2):123. PMC: 8907010. DOI: 10.1007/s00018-021-04092-2. View