Chemical Communication Between Giant Vesicles and Gated Nanoparticles for Strip-Based Sensing

Overview

Journal Nano Lett

Publisher American Chemical Society

Specialty Biotechnology

Date 2024 Oct 23

PMID 39442006

Authors

Jordi Ventura-Cobos

Estela Climent

Ramon Martinez-Manez

Antoni Llopis-Lorente

Affiliations

Soon will be listed here.

Abstract

Inspired by nature, the development of artificial micro/nanosystems capable of communicating has become an emergent topic in nanotechnology, synthetic biology, and related areas. However, the demonstration of actual applications still has to come. Here, we demonstrate how chemical communication between micro- and nanoparticles can be used for the design of sensing systems. To realize this, we synergistically combine two different types of particles: i.e., giant unilamellar vesicles (GUVs) as senders and gated mesoporous nanoparticles as receivers. The use of engineered GUVs allows the detection of analytes based on responsive membranes, while the use of gated nanoparticles allows a straightforward application on test strips with smartphone-based detection. In addition, we demonstrate that the combined communication system exhibits signal amplification and its application in real samples employing the bacterial toxin α-hemolysin as target analyte. Altogether, our report presents a new route for engineering sensing systems based on the combination of communicative micro/nanoparticles.

References

Garrido E, Climent E, Marcos M, Sancenon F, Rurack K, Martinez-Manez R . Dualplex lateral flow assay for simultaneous scopolamine and "cannibal drug" detection based on receptor-gated mesoporous nanoparticles. Nanoscale. 2022; 14(37):13505-13513. DOI: 10.1039/d2nr03325a. View

Smith J, Hartmann D, Booth M . Engineering cellular communication between light-activated synthetic cells and bacteria. Nat Chem Biol. 2023; 19(9):1138-1146. PMC: 10449621. DOI: 10.1038/s41589-023-01374-7. View

Lee Y, Thompson D . Stimuli-responsive liposomes for drug delivery. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2017; 9(5). PMC: 5557698. DOI: 10.1002/wnan.1450. View

Rampioni G, DAngelo F, Messina M, Zennaro A, Kuruma Y, Tofani D . Synthetic cells produce a quorum sensing chemical signal perceived by Pseudomonas aeruginosa. Chem Commun (Camb). 2018; 54(17):2090-2093. DOI: 10.1039/c7cc09678j. View

van Meer G, Voelker D, Feigenson G . Membrane lipids: where they are and how they behave. Nat Rev Mol Cell Biol. 2008; 9(2):112-24. PMC: 2642958. DOI: 10.1038/nrm2330. View

Tang T, Cecchi D, Fracasso G, Accardi D, Coutable-Pennarun A, Mansy S . Gene-Mediated Chemical Communication in Synthetic Protocell Communities. ACS Synth Biol. 2017; 7(2):339-346. DOI: 10.1021/acssynbio.7b00306. View

Rampioni G, DAngelo F, Leoni L, Stano P . Gene-Expressing Liposomes as Synthetic Cells for Molecular Communication Studies. Front Bioeng Biotechnol. 2019; 7:1. PMC: 6344414. DOI: 10.3389/fbioe.2019.00001. View

Chen H, Xu W, Shi H, Qiao Y, He X, Zheng J . DNA-Based Artificial Receptors as Transmembrane Signal Transduction Systems for Protocellular Communication. Angew Chem Int Ed Engl. 2023; 62(23):e202301559. DOI: 10.1002/anie.202301559. View

Ariga K, Song J, Kawakami K . Layer-by-layer designer nanoarchitectonics for physical and chemical communications in functional materials. Chem Commun (Camb). 2024; 60(16):2152-2167. DOI: 10.1039/d3cc04952c. View

10.

Mashima T, van Stevendaal M, Cornelissens F, Mason A, Rosier B, Altenburg W . DNA-Mediated Protein Shuttling between Coacervate-Based Artificial Cells. Angew Chem Int Ed Engl. 2022; 61(17):e202115041. PMC: 9303767. DOI: 10.1002/anie.202115041. View

11.

Rideau E, Dimova R, Schwille P, Wurm F, Landfester K . Liposomes and polymersomes: a comparative review towards cell mimicking. Chem Soc Rev. 2018; 47(23):8572-8610. DOI: 10.1039/c8cs00162f. View

12.

Llopis-Lorente A, Buddingh B, Martinez-Manez R, van Hest J, Abdelmohsen L . Quorum sensing communication between lipid-based artificial cells. Chem Commun (Camb). 2022; 59(5):579-582. DOI: 10.1039/d2cc05367e. View

13.

Heidari A, Senturk O, Yang S, Joesaar A, Gobbo P, Mann S . Orthogonal Light-Dependent Membrane Adhesion Induces Social Self-Sorting and Member-Specific DNA Communication in Synthetic Cell Communities. Small. 2023; 19(13):e2206474. DOI: 10.1002/smll.202206474. View

14.

Tseng Y, Song J, Zhang J, Shandilya E, Sen A . Chemomechanical Communication between Liposomes Based on Enzyme Cascades. J Am Chem Soc. 2024; 146(23):16097-16104. DOI: 10.1021/jacs.4c03415. View

15.

Eyer P, Worek F, Kiderlen D, Sinko G, Stuglin A, Simeon-Rudolf V . Molar absorption coefficients for the reduced Ellman reagent: reassessment. Anal Biochem. 2003; 312(2):224-7. DOI: 10.1016/s0003-2697(02)00506-7. View

16.

Antoniou A, Giofre S, Seneci P, Passarella D, Pellegrino S . Stimulus-responsive liposomes for biomedical applications. Drug Discov Today. 2021; 26(8):1794-1824. DOI: 10.1016/j.drudis.2021.05.010. View

17.

Diez P, Sanchez A, de la Torre C, Gamella M, Martinez-Ruiz P, Aznar E . Neoglycoenzyme-Gated Mesoporous Silica Nanoparticles: Toward the Design of Nanodevices for Pulsatile Programmed Sequential Delivery. ACS Appl Mater Interfaces. 2016; 8(12):7657-65. DOI: 10.1021/acsami.5b12645. View

18.

Parolo C, Merkoci A . Paper-based nanobiosensors for diagnostics. Chem Soc Rev. 2012; 42(2):450-7. DOI: 10.1039/c2cs35255a. View

19.

Buddingh B, Elzinga J, van Hest J . Intercellular communication between artificial cells by allosteric amplification of a molecular signal. Nat Commun. 2020; 11(1):1652. PMC: 7125153. DOI: 10.1038/s41467-020-15482-8. View

20.

Hu X, Zhang Y, Xie Z, Jing X, Bellotti A, Gu Z . Stimuli-Responsive Polymersomes for Biomedical Applications. Biomacromolecules. 2017; 18(3):649-673. DOI: 10.1021/acs.biomac.6b01704. View