Spatiotemporal Analysis of F-actin Polymerization with Micropillar Arrays Reveals Synchronization Between Adhesion Sites

Overview

Journal Mol Biol Cell

Specialties Cell Biology
Molecular Biology

Date 2024 Oct 23

PMID 39441710

Authors

Sarit Hollander

Yuanning Guo

Haguy Wolfenson

Assaf Zaritsky

Affiliations

Soon will be listed here.

Abstract

We repurposed micropillar arrays to quantify spatiotemporal inter-adhesion communication. Following the observation that integrin adhesions formed around pillar tops we relied on the precise repetitive spatial control of the pillars to reliably monitor F-actin dynamics in mouse embryonic fibroblasts as a model for spatiotemporal adhesion-related intracellular signaling. Using correlation-based analyses, we revealed localized information flows propagating between adjacent pillars that were integrated over space and time to synchronize the adhesion dynamics within the entire cell. Probing the mechanical regulation, we discovered that stiffer pillars or partial actomyosin contractility inhibition enhances inter-adhesion F-actin synchronization, and that inhibition of Arp2/3, but not formin, reduces synchronization. Our results suggest that adhesions can communicate and highlight the potential of using micropillar arrays as a tool to measure spatiotemporal intracellular signaling.

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