Fabrication of Injectable, Adhesive, Self-healing, Superabsorbent Hydrogels Based on Quaternary Ammonium Chitosan and Oxidized Pullulan

Overview

Journal Heliyon

Specialty Social Sciences

Date 2024 Oct 22

PMID 39435091

Authors

Qian He

Xiaoyue Ding

Jun Deng

Yanze Zhang

Xiaoyi Wang

Dan Zhan

Oseweuba Valentine Okoro

Lizhao Yan

Armin Shavandi

Lei Nie

Affiliations

Soon will be listed here.

Abstract

Injectable hydrogels, which are polymeric materials that are characterized by their ability to be injected in a liquid form into cavities and subsequently undergo in situ solidification, have garnered significant attention. These materials are extensively used in a range of biomedical applications. This study synthesized several injectable composite hydrogels through the mild Schiff base reaction while imposing different concentrations of quaternary ammonium chitosan and oxidized pullulan. Subsequent characterizations revealed a consistent and coherent porous structure within the hydrogels with smooth inner walls. The hydrogels were also determined to possess good adhesion, mechanical properties, self-healing ability, and injectability. Furthermore, antimicrobial tests against and demonstrated antibacterial properties, which improved with increasing concentrations of quaternary ammonium chitosan. Co-culturing with skin fibroblasts demonstrated that the injectable hydrogels exhibited favourable biocompatibility and the capacity to boost cellular activity, thus underscoring its potential for use in biomedical applications.

References

Chen Z, Yao X, Liu L, Guan J, Liu M, Li Z . Blood coagulation evaluation of N-alkylated chitosan. Carbohydr Polym. 2017; 173:259-268. DOI: 10.1016/j.carbpol.2017.05.085. View

Andreica B, Mititelu-Tartau L, Rosca I, Pelin I, Nicol E, Marin L . Biocompatible hydrogels based on quaternary ammonium salts of chitosan with high antimicrobial activity as biocidal agents for disinfection. Carbohydr Polym. 2024; 342:122389. DOI: 10.1016/j.carbpol.2024.122389. View

Bertsch P, Diba M, Mooney D, Leeuwenburgh S . Self-Healing Injectable Hydrogels for Tissue Regeneration. Chem Rev. 2022; 123(2):834-873. PMC: 9881015. DOI: 10.1021/acs.chemrev.2c00179. View

Roy S, Halder M, Ramprasad P, Dasgupta S, Singh Y, Pal D . Oxidized pullulan exhibits potent antibacterial activity against S. aureus by disrupting its membrane integrity. Int J Biol Macromol. 2023; 249:126049. DOI: 10.1016/j.ijbiomac.2023.126049. View

Rai M, Wypij M, Ingle A, Trzcinska-Wencel J, Golinska P . Emerging Trends in Pullulan-Based Antimicrobial Systems for Various Applications. Int J Mol Sci. 2021; 22(24). PMC: 8704206. DOI: 10.3390/ijms222413596. View

Piegat A, Zywicka A, Niemczyk A, Goszczynska A . Antibacterial Activity of ,-Acylated Chitosan Derivative. Polymers (Basel). 2021; 13(1). PMC: 7794783. DOI: 10.3390/polym13010107. View

Sumayya A, Kurup G . Marine macromolecules cross-linked hydrogel scaffolds as physiochemically and biologically favorable entities for tissue engineering applications. J Biomater Sci Polym Ed. 2017; 28(9):807-825. DOI: 10.1080/09205063.2017.1303119. View

Jeznach O, Kolbuk D, Sajkiewicz P . Injectable hydrogels and nanocomposite hydrogels for cartilage regeneration. J Biomed Mater Res A. 2018; 106(10):2762-2776. DOI: 10.1002/jbm.a.36449. View

Bhardwaj G, Webster T . Increased NIH 3T3 fibroblast functions on cell culture dishes which mimic the nanometer fibers of natural tissues. Int J Nanomedicine. 2015; 10:5293-9. PMC: 4554417. DOI: 10.2147/IJN.S83007. View

10.

Ding P, Ding X, Li J, Guo W, Okoro O, Mirzaei M . Facile preparation of self-healing hydrogels based on chitosan and PVA with the incorporation of curcumin-loaded micelles for wound dressings. Biomed Mater. 2024; 19(2). DOI: 10.1088/1748-605X/ad1df9. View

11.

Correa S, Grosskopf A, Lopez Hernandez H, Chan D, Yu A, Stapleton L . Translational Applications of Hydrogels. Chem Rev. 2021; 121(18):11385-11457. PMC: 8461619. DOI: 10.1021/acs.chemrev.0c01177. View

12.

Nie L, Wei Q, Sun M, Ding P, Wang L, Sun Y . Injectable, self-healing, transparent, and antibacterial hydrogels based on chitosan and dextran for wound dressings. Int J Biol Macromol. 2023; 233:123494. DOI: 10.1016/j.ijbiomac.2023.123494. View

13.

Qian C, Zhang T, Gravesande J, Baysah C, Song X, Xing J . Injectable and self-healing polysaccharide-based hydrogel for pH-responsive drug release. Int J Biol Macromol. 2018; 123:140-148. DOI: 10.1016/j.ijbiomac.2018.11.048. View

14.

Xuan H, Wu S, Fei S, Li B, Yang Y, Yuan H . Injectable nanofiber-polysaccharide self-healing hydrogels for wound healing. Mater Sci Eng C Mater Biol Appl. 2021; 128:112264. DOI: 10.1016/j.msec.2021.112264. View

15.

Fan L, Zou S, Ge H, Xiao Y, Wen H, Feng H . Preparation and characterization of hydroxypropyl chitosan modified with collagen peptide. Int J Biol Macromol. 2016; 93(Pt A):636-643. DOI: 10.1016/j.ijbiomac.2016.07.093. View

16.

Wang L, Ding X, Li J, Li M, Ding P, Guo W . Genipin crosslinked quaternary ammonium chitosan hydrogels for wound dressings. Biomed Mater. 2024; 19(4). DOI: 10.1088/1748-605X/ad525f. View

17.

Wu L, Chen G, Li Z . Layered Rare-Earth Hydroxide/Polyacrylamide Nanocomposite Hydrogels with Highly Tunable Photoluminescence. Small. 2017; 13(23). DOI: 10.1002/smll.201604070. View

18.

Yin X, Tsukaya H . A pulse-chase strategy for EdU labelling assay is able to rapidly quantify cell division orientation. New Phytol. 2016; 211(4):1462-9. DOI: 10.1111/nph.13980. View

19.

Sun Z, Shi C, Wang X, Fang Q, Huang J . Synthesis, characterization, and antimicrobial activities of sulfonated chitosan. Carbohydr Polym. 2016; 155:321-328. DOI: 10.1016/j.carbpol.2016.08.069. View

20.

Peers S, Montembault A, Ladaviere C . Chitosan hydrogels for sustained drug delivery. J Control Release. 2020; 326:150-163. DOI: 10.1016/j.jconrel.2020.06.012. View