GLO1 Regulates Hepatocellular Carcinoma Proliferation and Migration Through the Cell Cycle Pathway

Overview

Journal BMC Cancer

Publisher Biomed Central

Specialty Oncology

Date 2024 Oct 21

PMID 39434012

Authors

Yao Zhang

Xiaolong Tang

Lin Liu

Dan Cai

Shuang Gou

Siyu Hao

Yan Li

Jing Shen

Yu Chen

Yueshui Zhao

Xu Wu

Mingxing Li

Meijuan Chen

Xiaobing Li

Yuhong Sun

Li Gu

Wanping Li

Fang Wang

Zhuo Zhang

Xiaodong Wang

Shuai Deng

Zhangang Xiao

Lei Yao

Fukuan Du

Affiliations

Soon will be listed here.

Abstract

Background: Hepatocellular carcinoma (HCC) is a malignant tumor characterized by a high mortality rate. The occurrence and progression of HCC are linked to oxidative stress. Glyoxalase-1 (GLO1) plays an important role in regulating oxidative stress, yet the underlying mechanism remains unclear. GLO1 may serve as a prognostic biomarker and therapeutic target for HCC.

Methods: Based on TCGA database hepatocellular carcinoma samples, we conducted a bioinformatics analysis to explore the correlation between GLO1 expression and HCC cell proliferation and viability. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis revealed that differentially expressed genes (DEGs) were mainly enriched in the cell cycle pathway. We analyzed the relationships between GLO1 and 24 genes enriched in the cell cycle pathway using a protein-protein interaction (PPI) network. Finally, experimental validation was performed to assess GLO1's impact on the distribution of cells at different cell cycle stages and on the proliferation and migration of HCC cells.

Results: Our study demonstrated that GLO1 was overexpressed in HCC tissues and was associated with a poor prognosis. Data analysis indicated that overexpression of GLO1 activated the cell cycle pathway and positively correlated with expression of the majority of key cell cycle genes. Experimental validation showed that GLO1 expression affects the number of HCC cells in G2 and S phases and regulates HCC cell proliferation and migration.

Conclusions: GLO1 represents a promising therapeutic target for HCC, providing valuable insights into its role in the viability and proliferation of HCC cells.

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