Exploring Tc-Labeled Iron-Binding Glycoprotein Nanoparticles As a Potential Nanoplatform for Sentinel Lymph Node Imaging: Development, Characterization, and Radiolabeling Studies

Overview

Journal ACS Omega

Publisher American Chemical Society

Specialty Chemistry

Date 2024 Oct 21

PMID 39431106

Authors

Sanjay Kulkarni

Anuj Kumar

Abhijeet Pandey

Soji Soman

Suresh Subramanian

Srinivas Mutalik

Affiliations

Soon will be listed here.

Abstract

Lactoferrin, an iron binding glycoprotein-based nanoparticle, has emerged as a promising platform for drug delivery and imaging. This study presents the potential use of the protein nanocarrier in tracking sentinel lymph nodes for cancer staging. Lactoferrin nanoparticles (LF-NPs) were synthesized using a thermal treatment process and optimized to obtain 60-70 nm particle size with PDI less than 0.2. The NPs were characterized microscopically and spectroscopically, ensuring a comprehensive understanding of their physicochemical properties. The LF-NPs were found to be stable in different pH conditions. Their biocompatibility was confirmed through cytotoxicity assessments on RAW 264.7 cells, and hemolysis assay and in vivo toxicity study reveal their safe profile. Additionally, LF-NPs were successfully radiolabeled with technetium-99m (>90% labeling yield). Cell uptake studies with RAW 264.7 exhibited an uptake of ∼6%. Biodistribution studies in Wistar rats shed light on their in vivo behavior and suitability for targeted drug delivery systems. These findings collectively emphasize the multifaceted utility of LF-NPs, positioning them as a promising platform for diverse biomedical innovations.

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