Development of Multiplex Real-time PCR for Simultaneous Detection of Common Fungal Pathogens in Invasive Mycoses

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2024 Oct 21

PMID 39430554

Authors

Yasmin Khairani Muhammad Ismadi

Suharni Mohamad

Azian Harun

Affiliations

Soon will be listed here.

Abstract

Background: Fungi are common opportunistic pathogens that pose a significant threat to immunocompromised patients, particularly when late detection occurs.

Methods: In this study a multiplex real-time PCR has been developed for simultaneous detection of common fungal pathogens associated with invasive mycoses in a diagnostic setting.

Results: The specificity of the assay was rigorously tested on 40 types of organisms ( = 65), demonstrating 100% specificity. The limit of detection was determined to be 100 pg/μl (10 copies/μl), achievable within a rapid 3-h timeframe. The PCR assay efficiency exhibited a range between 89.77% and 104.30% for each target organism, with linearity falling between 0.9780 and 0.9983.

Conclusion: This multiplex real-time PCR assay holds promise for enhancing the timely and accurate diagnosis of invasive mycoses, particularly in immunocompromised patient populations.

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