Study of Sulfoglycolysis in Reveals a Widespread Bifurcated Pathway for Dihydroxypropanesulfonate Degradation

Overview

Journal iScience

Publisher Cell Press

Date 2024 Oct 21

PMID 39429772

Authors

Yiwei Chen

Ruoxing Chu

Kailiang Ma

Li Jiang

Qiaoyu Yang

Zhi Li

Min Hu

Qiuyi Guo

Fengxia Lu

Yifeng Wei

Yan Zhang

Yang Tong

Affiliations

Soon will be listed here.

Abstract

Sulfoquinovose (SQ), the polar head group of sulfolipids essential for photosynthesis, is naturally abundant. Anaerobic Firmicutes degrade SQ through a transaldolase-dependent (sulfo-TAL) pathway, producing dihydroxypropanesulfonate (DHPS). Some bacteria extend this pathway by the sequential action of HpfG and HpfD converting DHPS to 3-hydroxypropanesulfonate (3-HPS) via 3-sulfopropionaldehyde (3-SPA). Here, we report a variant sulfo-TAL pathway in , involving additional enzymes, a NAD-dependent 3-SPA dehydrogenase HpfX, and a 3-sulfopropionyl-CoA synthetase HpfYZ, which oxidize 3-SPA to 3-sulfopropionate (3-SP) coupled with ATP formation. grown on SQ or DHPS produced a mixture of 3-HPS and 3-SP, indicating the bifurcated pathway. Similar genes are found in various Firmicutes, including gut bacteria. Importantly, 3-SP, but not 3-HPS, can serve as a respiratory terminal electron acceptor for , a common intestinal pathobiont, resulting in the production of toxic HS. This research expands our understanding of sulfonate metabolism and reveals cross-feeding in the anaerobic microbiome.

Citing Articles

Anaerobic spp. degrade sulfoquinovose via a bifurcated 6-deoxy-6-sulfofructose transketolase/transaldolase pathway to both C- and C-sulfonate intermediates.

Borusak S, Denger K, Dorendorf T, Fournier C, Lerner H, Mayans O Front Microbiol. 2024; 15:1491101.

PMID: 39712897 PMC: 11659671. DOI: 10.3389/fmicb.2024.1491101.

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