Osteogenic Assessment of Leukocyte Platelet-rich Fibrin and Injectable Platelet-rich Fibrin in the Human Osteosarcoma MG - 63 Cell Line in Chronic Periodontitis Patients: An Study

Overview

Journal J Indian Soc Periodontol

Date 2024 Oct 16

PMID 39411735

Authors

A Sheryl Dolly

Prem Blaisie Rajula

P L Ravi Shankar

S Lochini

Mohamed Rashik

Sindhujaa Raja

Affiliations

Soon will be listed here.

Abstract

Background: The burgeoning interest in implant and regenerative dental care has led to a notable upsurge in the utilization of regenerative modalities. The intent of the present investigation was to evaluate the osteogenic ability of two different concentrated platelet groups at various concentrations in the human osteosarcoma MG-63 cell line.

Materials And Methods: Blood samples from 21 volunteers with chronic periodontitis were collected which were then centrifuged in accordance with the protocols of Choukroun . and Miron . to produce leukocyte- and platelet-rich fibrin (L-PRF) and injectable platelet-rich fibrin (I-PRF), respectively. Following MG-63 cell culture, the osteogenic ability of 0, 4%, and 20% concentrations of both L-PRF and I-PRF were determined using the real-time polymerase chain reaction assay.

Results: The results showed that 20% I-PRF (1.52 ± 0.24) and 4% L-PRF (1.42 ± 0.37) had the highest amount of bone morphogenetic protein 2 and osteocalcin, respectively.

Conclusion: I-PRF appears to promote the initial differentiation of cells.

References

Gassling V, Acil Y, Springer I, Hubert N, Wiltfang J . Platelet-rich plasma and platelet-rich fibrin in human cell culture. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009; 108(1):48-55. DOI: 10.1016/j.tripleo.2009.02.007. View

Gollapudi M, Bajaj P, Oza R . Injectable Platelet-Rich Fibrin - A Revolution in Periodontal Regeneration. Cureus. 2022; 14(8):e28647. PMC: 9525133. DOI: 10.7759/cureus.28647. View

Choukroun J, Ghanaati S . Reduction of relative centrifugation force within injectable platelet-rich-fibrin (PRF) concentrates advances patients' own inflammatory cells, platelets and growth factors: the first introduction to the low speed centrifugation concept. Eur J Trauma Emerg Surg. 2017; 44(1):87-95. PMC: 5808086. DOI: 10.1007/s00068-017-0767-9. View

Ross R, GLOMSET J, Kariya B, Harker L . A platelet-dependent serum factor that stimulates the proliferation of arterial smooth muscle cells in vitro. Proc Natl Acad Sci U S A. 1974; 71(4):1207-10. PMC: 388193. DOI: 10.1073/pnas.71.4.1207. View

Klein R, Fausti K, Carlos A . Ethanol inhibits human osteoblastic cell proliferation. Alcohol Clin Exp Res. 1996; 20(3):572-8. DOI: 10.1111/j.1530-0277.1996.tb01095.x. View

Fernandez-Medina T, Vaquette C, Ivanovski S . Systematic Comparison of the Effect of Four Clinical-Grade Platelet Rich Hemoderivatives on Osteoblast Behaviour. Int J Mol Sci. 2019; 20(24). PMC: 6941155. DOI: 10.3390/ijms20246243. View

Lee C, Chiu T, Chuang S, Tarnow D, Stoupel J . Alterations of the bone dimension following immediate implant placement into extraction socket: systematic review and meta-analysis. J Clin Periodontol. 2014; 41(9):914-26. DOI: 10.1111/jcpe.12276. View

Wang E, Rosen V, DAlessandro J, Bauduy M, Cordes P, Harada T . Recombinant human bone morphogenetic protein induces bone formation. Proc Natl Acad Sci U S A. 1990; 87(6):2220-4. PMC: 53658. DOI: 10.1073/pnas.87.6.2220. View

Katagiri T, Yamaguchi A, Ikeda T, Yoshiki S, Wozney J, Rosen V . The non-osteogenic mouse pluripotent cell line, C3H10T1/2, is induced to differentiate into osteoblastic cells by recombinant human bone morphogenetic protein-2. Biochem Biophys Res Commun. 1990; 172(1):295-9. DOI: 10.1016/s0006-291x(05)80208-6. View

10.

Zhang J, Yin C, Zhao Q, Zhao Z, Wang J, Miron R . Anti-inflammation effects of injectable platelet-rich fibrin via macrophages and dendritic cells. J Biomed Mater Res A. 2019; 108(1):61-68. DOI: 10.1002/jbm.a.36792. View

11.

Marrelli M, Tatullo M . Influence of PRF in the healing of bone and gingival tissues. Clinical and histological evaluations. Eur Rev Med Pharmacol Sci. 2013; 17(14):1958-62. View

12.

Bendall A, Abate-Shen C . Roles for Msx and Dlx homeoproteins in vertebrate development. Gene. 2000; 247(1-2):17-31. DOI: 10.1016/s0378-1119(00)00081-0. View

13.

Franceschi R, James W, Zerlauth G . 1 alpha, 25-dihydroxyvitamin D3 specific regulation of growth, morphology, and fibronectin in a human osteosarcoma cell line. J Cell Physiol. 1985; 123(3):401-9. DOI: 10.1002/jcp.1041230316. View

14.

Tan W, Wong T, Wong M, Lang N . A systematic review of post-extractional alveolar hard and soft tissue dimensional changes in humans. Clin Oral Implants Res. 2012; 23 Suppl 5:1-21. DOI: 10.1111/j.1600-0501.2011.02375.x. View

15.

Horowitz R, Holtzclaw D, Rosen P . A review on alveolar ridge preservation following tooth extraction. J Evid Based Dent Pract. 2012; 12(3 Suppl):149-60. DOI: 10.1016/S1532-3382(12)70029-5. View

16.

Beck Jr G . Inorganic phosphate as a signaling molecule in osteoblast differentiation. J Cell Biochem. 2003; 90(2):234-43. DOI: 10.1002/jcb.10622. View

17.

Farshidfar N, Amiri M, Jafarpour D, Hamedani S, Niknezhad S, Tayebi L . The feasibility of injectable PRF (I-PRF) for bone tissue engineering and its application in oral and maxillofacial reconstruction: From bench to chairside. Biomater Adv. 2022; 134:112557. PMC: 9295636. DOI: 10.1016/j.msec.2021.112557. View

18.

Esmaeilnejad A, Talebi Ardakani M, Shokri M, Nima Hosseini Khou P, Kamani M . Comparative Evaluation of the Effect of Two Platelet Concentrates (a-PRF and L-PRF) on the Cellular Activity of Pre-osteoblastic MG-63 Cell Line: An in vitro Study. J Dent (Shiraz). 2023; 24(2):235-244. PMC: 10300147. DOI: 10.30476/dentjods.2022.93305.1709. View

19.

Dohan D, Choukroun J, Diss A, Dohan S, Dohan A, Mouhyi J . Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006; 101(3):e37-44. DOI: 10.1016/j.tripleo.2005.07.008. View

20.

Lee J, Kim M, Byun J, Kim G, Ro J, Hwang D . The effect of biomechanical stimulation on osteoblast differentiation of human jaw periosteum-derived stem cells. Maxillofac Plast Reconstr Surg. 2017; 39(1):7. PMC: 5337228. DOI: 10.1186/s40902-017-0104-6. View