Callose and Salicylic Acid Are Key Determinants of Strigolactone-Mediated Disease Resistance in Arabidopsis

Overview

Journal Plants (Basel)

Date 2024 Oct 16

PMID 39409636

Authors

Xiaosheng Zhao

Qiuping Liu

Leitao Tan

Affiliations

Soon will be listed here.

Abstract

Research has demonstrated that strigolactones (SLs) mediate plant disease resistance; however, the basal mechanism is unclear. Here, we provide key genetic evidence supporting how SLs mediate plant disease resistance. Exogenous application of the SL analog, -GR24, increased resistance to virulent . SL-biosynthetic mutants and overexpression lines of more axillary growth 1 (, an SL-biosynthetic gene) enhanced and reduced bacterial susceptibility, respectively. In addition, -GR24 promoted bacterial pattern flg22-induced callose deposition and hydrogen peroxide production. SL-biosynthetic mutants displayed reduced callose deposition but not hydrogen peroxide production under flg22 treatment. Moreover, -GR24 did not affect avirulent effector-induced cell death between Col-0 and SL-biosynthetic mutants. Furthermore, -GR24 increased the free salicylic acid (SA) content and significantly promoted the expression of pathogenesis-related gene 1 related to SA signaling. Importantly, -GR24- and -induced bacterial resistance disappeared completely in Arabidopsis plants lacking both callose synthase and SA. Taken together, our data revealed that callose and SA are two important determinants in SL-mediated plant disease resistance, at least in Arabidopsis.

References

Chen J, Yang H, Ma S, Yao R, Huang X, Yan J . HbCOI1 perceives jasmonate to trigger signal transduction in Hevea brasiliensis. Tree Physiol. 2020; 41(3):460-471. DOI: 10.1093/treephys/tpaa124. View

Thomma B, Eggermont K, Penninckx I, Mauch-Mani B, Vogelsang R, Cammue B . Separate jasmonate-dependent and salicylate-dependent defense-response pathways in Arabidopsis are essential for resistance to distinct microbial pathogens. Proc Natl Acad Sci U S A. 1998; 95(25):15107-11. PMC: 24583. DOI: 10.1073/pnas.95.25.15107. View

Nasir F, Tian L, Shi S, Chang C, Ma L, Gao Y . Strigolactones positively regulate defense against Magnaporthe oryzae in rice (Oryza sativa). Plant Physiol Biochem. 2019; 142:106-116. DOI: 10.1016/j.plaphy.2019.06.028. View

Piisila M, Keceli M, Brader G, Jakobson L, Joesaar I, Sipari N . The F-box protein MAX2 contributes to resistance to bacterial phytopathogens in Arabidopsis thaliana. BMC Plant Biol. 2015; 15:53. PMC: 4340836. DOI: 10.1186/s12870-015-0434-4. View

Hanahan D . Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983; 166(4):557-80. DOI: 10.1016/s0022-2836(83)80284-8. View

Booker J, Auldridge M, Wills S, McCarty D, Klee H, Leyser O . MAX3/CCD7 is a carotenoid cleavage dioxygenase required for the synthesis of a novel plant signaling molecule. Curr Biol. 2004; 14(14):1232-8. DOI: 10.1016/j.cub.2004.06.061. View

Hauck P, Thilmony R, He S . A Pseudomonas syringae type III effector suppresses cell wall-based extracellular defense in susceptible Arabidopsis plants. Proc Natl Acad Sci U S A. 2003; 100(14):8577-82. PMC: 166271. DOI: 10.1073/pnas.1431173100. View

Nishimura M, Stein M, Hou B, Vogel J, Edwards H, Somerville S . Loss of a callose synthase results in salicylic acid-dependent disease resistance. Science. 2003; 301(5635):969-72. DOI: 10.1126/science.1086716. View

Auldridge M, Block A, Vogel J, Dabney-Smith C, Mila I, Bouzayen M . Characterization of three members of the Arabidopsis carotenoid cleavage dioxygenase family demonstrates the divergent roles of this multifunctional enzyme family. Plant J. 2006; 45(6):982-93. DOI: 10.1111/j.1365-313X.2006.02666.x. View

10.

Mayzlish-Gati E, De-Cuyper C, Goormachtig S, Beeckman T, Vuylsteke M, Brewer P . Strigolactones are involved in root response to low phosphate conditions in Arabidopsis. Plant Physiol. 2012; 160(3):1329-41. PMC: 3490576. DOI: 10.1104/pp.112.202358. View

11.

Lazar G, Goodman H . MAX1, a regulator of the flavonoid pathway, controls vegetative axillary bud outgrowth in Arabidopsis. Proc Natl Acad Sci U S A. 2006; 103(2):472-6. PMC: 1324789. DOI: 10.1073/pnas.0509463102. View

12.

Changenet V, Macadre C, Boutet-Mercey S, Magne K, Januario M, Dalmais M . Overexpression of a Cytochrome P450 Monooxygenase Involved in Orobanchol Biosynthesis Increases Susceptibility to Fusarium Head Blight. Front Plant Sci. 2021; 12:662025. PMC: 8048717. DOI: 10.3389/fpls.2021.662025. View

13.

Dong X . SA, JA, ethylene, and disease resistance in plants. Curr Opin Plant Biol. 1999; 1(4):316-23. DOI: 10.1016/1369-5266(88)80053-0. View

14.

Umehara M, Hanada A, Yoshida S, Akiyama K, Arite T, Takeda-Kamiya N . Inhibition of shoot branching by new terpenoid plant hormones. Nature. 2008; 455(7210):195-200. DOI: 10.1038/nature07272. View

15.

KING E, Ward M, RANEY D . Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med. 1954; 44(2):301-7. View

16.

Rozpadek P, Domka A, Nosek M, Wazny R, Jedrzejczyk R, Wiciarz M . The Role of Strigolactone in the Cross-Talk Between and the Endophytic Fungus sp. Front Microbiol. 2018; 9:441. PMC: 5867299. DOI: 10.3389/fmicb.2018.00441. View

17.

Koltai H, Kapulnik Y . Strigolactones as mediators of plant growth responses to environmental conditions. Plant Signal Behav. 2011; 6(1):37-41. PMC: 3122003. DOI: 10.4161/psb.6.1.13245. View

18.

Mei S, Hou S, Cui H, Feng F, Rong W . Characterization of the interaction between Oidium heveae and Arabidopsis thaliana. Mol Plant Pathol. 2016; 17(9):1331-1343. PMC: 6638524. DOI: 10.1111/mpp.12363. View

19.

Kusajima M, Fujita M, Soudthedlath K, Nakamura H, Yoneyama K, Nomura T . Strigolactones Modulate Salicylic Acid-Mediated Disease Resistance in . Int J Mol Sci. 2022; 23(9). PMC: 9101170. DOI: 10.3390/ijms23095246. View

20.

Wen S, Tu Z, Wei L, Li H . Liriodendron chinense LcMAX1 regulates primary root growth and shoot branching in Arabidopsis thaliana. Plant Physiol Biochem. 2022; 190:1-10. DOI: 10.1016/j.plaphy.2022.08.020. View