27-Hydroxycholesterol Negatively Affects the Function of Bone Marrow Endothelial Cells in the Bone Marrow

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Oct 16

PMID 39408846

Authors

Soo-Yeon Woo

Wan-Seog Shim

Hyejin Lee

Ninib Baryawno

Parkyong Song

Byoung Soo Kim

Sik Yoon

Sae-Ock Oh

Dongjun Lee

Affiliations

Soon will be listed here.

Abstract

Hematopoietic stem cells (HSCs) reside in specific microenvironments that facilitate their regulation through both internal mechanisms and external cues. Bone marrow endothelial cells (BMECs), which are found in one of these microenvironments, play a vital role in controlling the self-renewal and differentiation of HSCs during hematological stress. We previously showed that 27-hydroxycholesterol (27HC) administration of exogenous 27HC negatively affected the population of HSCs and progenitor cells by increasing the reactive oxygen species levels in the bone marrow. However, the effect of 27HC on BMECs is unclear. To determine the function of 27HC in BMECs, we employed magnetic-activated cell sorting to isolate CD31 BMECs and CD31 cells. We demonstrated the effect of 27HC on CD31 BMECs and HSCs. Treatment with exogenous 27HC led to a decrease in the number of BMECs and reduced the expression of adhesion molecules that are crucial for maintaining HSCs. Our results demonstrate that BMECs are sensitively affected by 27HC and are crucial for HSC survival.

References

Liu L, Li M, Xing Y, Wang X, Wang Y . The oncogenic roles of 27-hydroxycholesterol in glioblastoma. Oncol Lett. 2019; 18(4):3623-3629. PMC: 6757262. DOI: 10.3892/ol.2019.10690. View

Ashok D, Polcik L, Dannewitz Prosseda S, Hartmann T . Insights Into Bone Marrow Niche Stability: An Adhesion and Metabolism Route. Front Cell Dev Biol. 2022; 9:798604. PMC: 8806031. DOI: 10.3389/fcell.2021.798604. View

Butler J, Nolan D, Vertes E, Varnum-Finney B, Kobayashi H, Hooper A . Endothelial cells are essential for the self-renewal and repopulation of Notch-dependent hematopoietic stem cells. Cell Stem Cell. 2010; 6(3):251-64. PMC: 2866527. DOI: 10.1016/j.stem.2010.02.001. View

Greenbaum A, Hsu Y, Day R, Schuettpelz L, Christopher M, Borgerding J . CXCL12 in early mesenchymal progenitors is required for haematopoietic stem-cell maintenance. Nature. 2013; 495(7440):227-30. PMC: 3600148. DOI: 10.1038/nature11926. View

Baryawno N, Przybylski D, Kowalczyk M, Kfoury Y, Severe N, Gustafsson K . A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia. Cell. 2019; 177(7):1915-1932.e16. PMC: 6570562. DOI: 10.1016/j.cell.2019.04.040. View

Doan P, Chute J . The vascular niche: home for normal and malignant hematopoietic stem cells. Leukemia. 2011; 26(1):54-62. DOI: 10.1038/leu.2011.236. View

Kim J, Lee H, Hong C, Nam J, Yeo H, Cho W . Novel endogenous endoplasmic reticulum transmembrane protein SURF4 suppresses cell death by negatively regulating the STING-STAT6 axis in myeloid leukemia. Cancer Commun (Lond). 2022; 43(3):395-399. PMC: 10009663. DOI: 10.1002/cac2.12390. View

Woo S, Lee H, Park S, Choi H, Kim J, Kwon M . Role of reactive oxygen species in regulating 27-hydroxycholesterol-induced apoptosis of hematopoietic progenitor cells and myeloid cell lines. Cell Death Dis. 2022; 13(10):916. PMC: 9622808. DOI: 10.1038/s41419-022-05360-0. View

Asada N, Kunisaki Y, Pierce H, Wang Z, Fernandez N, Birbrair A . Differential cytokine contributions of perivascular haematopoietic stem cell niches. Nat Cell Biol. 2017; 19(3):214-223. PMC: 5467892. DOI: 10.1038/ncb3475. View

10.

Rettig M, Ansstas G, DiPersio J . Mobilization of hematopoietic stem and progenitor cells using inhibitors of CXCR4 and VLA-4. Leukemia. 2011; 26(1):34-53. PMC: 3514440. DOI: 10.1038/leu.2011.197. View

11.

Mendelson A, Frenette P . Hematopoietic stem cell niche maintenance during homeostasis and regeneration. Nat Med. 2014; 20(8):833-46. PMC: 4459580. DOI: 10.1038/nm.3647. View

12.

Adler B, Kaushansky K, Rubin C . Obesity-driven disruption of haematopoiesis and the bone marrow niche. Nat Rev Endocrinol. 2014; 10(12):737-48. DOI: 10.1038/nrendo.2014.169. View

13.

Liu Y, Zhang S, Chen Y, Shi K, Zou B, Liu J . ICAM-1 Deficiency in the Bone Marrow Niche Impairs Quiescence and Repopulation of Hematopoietic Stem Cells. Stem Cell Reports. 2018; 11(1):258-273. PMC: 6117479. DOI: 10.1016/j.stemcr.2018.05.016. View

14.

Lertkiatmongkol P, Liao D, Mei H, Hu Y, Newman P . Endothelial functions of platelet/endothelial cell adhesion molecule-1 (CD31). Curr Opin Hematol. 2016; 23(3):253-9. PMC: 4986701. DOI: 10.1097/MOH.0000000000000239. View

15.

Vurusaner B, Gargiulo S, Testa G, Gamba P, Leonarduzzi G, Poli G . The role of autophagy in survival response induced by 27-hydroxycholesterol in human promonocytic cells. Redox Biol. 2018; 17:400-410. PMC: 5986166. DOI: 10.1016/j.redox.2018.05.010. View

16.

Raza S, Meyer M, Schommer J, Hammer K, Guo B, Ghribi O . 27-Hydroxycholesterol stimulates cell proliferation and resistance to docetaxel-induced apoptosis in prostate epithelial cells. Med Oncol. 2016; 33(2):12. DOI: 10.1007/s12032-015-0725-5. View

17.

Nakada D, Oguro H, Levi B, Ryan N, Kitano A, Saitoh Y . Oestrogen increases haematopoietic stem-cell self-renewal in females and during pregnancy. Nature. 2014; 505(7484):555-8. PMC: 4015622. DOI: 10.1038/nature12932. View

18.

Baek A, Yu Y, He S, Wardell S, Chang C, Kwon S . The cholesterol metabolite 27 hydroxycholesterol facilitates breast cancer metastasis through its actions on immune cells. Nat Commun. 2017; 8(1):864. PMC: 5636879. DOI: 10.1038/s41467-017-00910-z. View

19.

Himburg H, Termini C, Schlussel L, Kan J, Li M, Zhao L . Distinct Bone Marrow Sources of Pleiotrophin Control Hematopoietic Stem Cell Maintenance and Regeneration. Cell Stem Cell. 2018; 23(3):370-381.e5. PMC: 6482945. DOI: 10.1016/j.stem.2018.07.003. View

20.

Russell D . The enzymes, regulation, and genetics of bile acid synthesis. Annu Rev Biochem. 2003; 72:137-74. DOI: 10.1146/annurev.biochem.72.121801.161712. View