Insight into the New Infection Pathway Resulting from Above-ground Pathogen Infection of Grapevine Crown Gall

Overview

Journal Front Plant Sci

Date 2024 Oct 15

PMID 39403616

Authors

Akira Kawaguchi

Affiliations

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Abstract

Grapevine crown gall (GCG), a soil-borne plant disease caused by tumorigenic (TAV) (=tumorigenic ) strains, poses a significant threat to grapevines worldwide. Recently, outbreaks of GCG have been reported in several vineyards, necessitating investigation into potential alternative infection pathways beyond soil transmission. The spatiotemporal distribution of GCG in vineyards from 2020 to 2022 was analyzed using the binary power law (BPL) model, with variations in quadrat shapes. Both total and newly observed diseased plants exhibited an aggregated distribution, indicating that new infections clustered around existing diseased plants, with secondary infections appearing as independent cluster points. This study provides evidence that infected pruning tools can transmit the pathogen to healthy grapevines and that TAV inoculation through spraying contributes more to GCG incidence than planting in infected soil alone. This represents the first documented case of secondary above-ground TAV infection contributing to GCG in commercial vineyards.

References

Burr T, Otten L . CROWN GALL OF GRAPE: Biology and Disease Management. Annu Rev Phytopathol. 2001; 37:53-80. DOI: 10.1146/annurev.phyto.37.1.53. View

Mousavi S, Willems A, Nesme X, De Lajudie P, Lindstrom K . Revised phylogeny of Rhizobiaceae: proposal of the delineation of Pararhizobium gen. nov., and 13 new species combinations. Syst Appl Microbiol. 2015; 38(2):84-90. DOI: 10.1016/j.syapm.2014.12.003. View

Burr T, Bazzi C, Sule S, Otten L . Crown Gall of Grape: Biology of Agrobacterium vitis and the Development of Disease Control Strategies. Plant Dis. 2019; 82(12):1288-1297. DOI: 10.1094/PDIS.1998.82.12.1288. View

Kawaguchi A, Inoue K, Tanina K . Evaluation of the Nonpathogenic Agrobacterium vitis Strain ARK-1 for Crown Gall Control in Diverse Plant Species. Plant Dis. 2019; 99(3):409-414. DOI: 10.1094/PDIS-06-14-0588-RE. View

Peritore-Galve F, Tancos M, Smart C . Bacterial Canker of Tomato: Revisiting a Global and Economically Damaging Seedborne Pathogen. Plant Dis. 2020; 105(6):1581-1595. DOI: 10.1094/PDIS-08-20-1732-FE. View

Kawaguchi A . Biological control of crown gall on grapevine and root colonization by nonpathogenic Rhizobium vitis strain ARK-1. Microbes Environ. 2013; 28(3):306-11. PMC: 4070965. DOI: 10.1264/jsme2.me13014. View

Madden L, Hughes G, Moraes W, Xu X, Turechek W . Twenty-Five Years of the Binary Power Law for Characterizing Heterogeneity of Disease Incidence. Phytopathology. 2017; 108(6):656-680. DOI: 10.1094/PHYTO-07-17-0234-RVW. View

Kawaguchi A, Inoue K, Ichinose Y . Biological control of crown gall of grapevine, rose, and tomato by nonpathogenic Agrobacterium vitis strain VAR03-1. Phytopathology. 2008; 98(11):1218-25. DOI: 10.1094/PHYTO-98-11-1218. View

Noutoshi Y, Toyoda A, Ishii T, Saito K, Watanabe M, Kawaguchi A . Complete Genome Sequence Data of Nonpathogenic Strain VAR03-1, a Biological Control Agent for Grapevine Crown Gall Disease. Mol Plant Microbe Interact. 2020; 33(12):1451-1453. DOI: 10.1094/MPMI-07-20-0181-A. View

10.

Gan H, Szegedi E, Fersi R, Chebil S, Kovacs L, Kawaguchi A . Insight Into the Microbial Co-occurrence and Diversity of 73 Grapevine () Crown Galls Collected Across the Northern Hemisphere. Front Microbiol. 2019; 10:1896. PMC: 6700373. DOI: 10.3389/fmicb.2019.01896. View

11.

Kawaguchi A, Nita M, Ishii T, Watanabe M, Noutoshi Y . Biological control agent Rhizobium (=Agrobacterium) vitis strain ARK-1 suppresses expression of the essential and non-essential vir genes of tumorigenic R. vitis. BMC Res Notes. 2019; 12(1):1. PMC: 6317203. DOI: 10.1186/s13104-018-4038-6. View

12.

Kawaguchi A . Reduction in pathogen populations at grapevine wound sites is associated with the mechanism underlying the biological control of crown gall by rhizobium vitis strain ARK-1. Microbes Environ. 2014; 29(3):296-302. PMC: 4159041. DOI: 10.1264/jsme2.me14059. View

13.

Ishii T, Tsuchida N, Hemelda N, Saito K, Bao J, Watanabe M . Rhizoviticin is an alphaproteobacterial tailocin that mediates biocontrol of grapevine crown gall disease. ISME J. 2024; 18(1). PMC: 10811719. DOI: 10.1093/ismejo/wrad003. View

14.

Kawaguchi A, Sone T, Ochi S, Matsushita Y, Noutoshi Y, Nita M . Origin of Pathogens of Grapevine Crown Gall Disease in Hokkaido in Japan as Characterized by Molecular Epidemiology of Strains. Life (Basel). 2021; 11(11). PMC: 8620909. DOI: 10.3390/life11111265. View

15.

Kawaguchi A, Kirino N, Inoue K . Biological Control for Grapevine Crown Gall Evaluated by a Network Meta-Analysis. Plants (Basel). 2023; 12(3). PMC: 9921260. DOI: 10.3390/plants12030572. View

16.

Kawaguchi A, Nemoto M, Ochi S, Matsushita Y, Sato T, Sone T . Insight into the population dynamics of pathogenic bacteria causing grapevine crown gall in snowfall areas: snow cover protects the proliferation of pathogenic bacteria. Front Plant Sci. 2023; 14:1198710. PMC: 10338858. DOI: 10.3389/fpls.2023.1198710. View

17.

Kawaguchi A, Inoue K, Tanina K, Nita M . Biological control for grapevine crown gall using nonpathogenic Rhizobium vitis strain ARK-1. Proc Jpn Acad Ser B Phys Biol Sci. 2017; 93(8):547-560. PMC: 5743857. DOI: 10.2183/pjab.93.035. View