Serum Exosomal Small Nucleolar RNA (snoRNA) Signatures As a Predictive Biomarker for Benign and Malignant Pulmonary Nodules

Overview

Journal Cancer Cell Int

Publisher Biomed Central

Date 2024 Oct 14

PMID 39402628

Authors

Fei Cao

Qian You

Feng Zhu

Yu Zhang

Affiliations

Soon will be listed here.

Abstract

Low-dose CT (LDCT) is increasingly recognized as the preferred method for detecting pulmonary nodules. However, distinguishing whether a nodule is benign or malignant often necessitates repeated scans or invasive tissue sampling procedures. Therefore, there is a pressing need for non-invasive techniques to minimize unnecessary interventions. This study aim to investigate the expression profile of exosomal snoRNA in the serum of patients with benign and malignant pulmonary nodules. We identified a total of 278 snoRNAs in serum exosomes, revealing significant differences in snoRNA levels between patients with malignant and benign nodules. Specifically, the upregulated snoRNAs U78 and U37 were validated through qRT-PCR and were found significantly elevated in the serum of patients with malignant pulmonary nodules, positioning them as promising biomarkers for the early detection of lung cancer. This study underscores the potential of serum exosomal U78 and U37 as critical tools for assessing the risk of pulmonary nodules identified through CT screening.

References

Peffers M, Chabronova A, Balaskas P, Fang Y, Dyer P, Cremers A . SnoRNA signatures in cartilage ageing and osteoarthritis. Sci Rep. 2020; 10(1):10641. PMC: 7326970. DOI: 10.1038/s41598-020-67446-z. View

Kuang M, Tao X, Peng Y, Zhang W, Pan Y, Cheng L . Proteomic analysis of plasma exosomes to differentiate malignant from benign pulmonary nodules. Clin Proteomics. 2019; 16:5. PMC: 6359787. DOI: 10.1186/s12014-019-9225-5. View

Redberg R, OMalley P . Important Questions About Lung Cancer Screening Programs When Incidental Findings Exceed Lung Cancer Nodules by 40 to 1. JAMA Intern Med. 2017; 177(3):311-312. DOI: 10.1001/jamainternmed.2016.9446. View

Rai A, Rajan K, Bisserier M, Brojakowska A, Sebastian A, Evans A . Spaceflight-Associated Changes of snoRNAs in Peripheral Blood Mononuclear Cells and Plasma Exosomes-A Pilot Study. Front Cardiovasc Med. 2022; 9:886689. PMC: 9267956. DOI: 10.3389/fcvm.2022.886689. View

Tang G, Zeng Z, Sun W, Li S, You C, Tang F . Small Nucleolar RNA 71A Promotes Lung Cancer Cell Proliferation, Migration and Invasion via MAPK/ERK Pathway. J Cancer. 2019; 10(10):2261-2275. PMC: 6584411. DOI: 10.7150/jca.31077. View

Kiss T . Small nucleolar RNAs: an abundant group of noncoding RNAs with diverse cellular functions. Cell. 2002; 109(2):145-8. DOI: 10.1016/s0092-8674(02)00718-3. View

Crossland R, Norden J, Bibby L, Davis J, Dickinson A . Evaluation of optimal extracellular vesicle small RNA isolation and qRT-PCR normalisation for serum and urine. J Immunol Methods. 2016; 429:39-49. DOI: 10.1016/j.jim.2015.12.011. View

Dong X, Song X, Ding S, Yu M, Shang X, Wang K . Tumor-educated platelet SNORD55 as a potential biomarker for the early diagnosis of non-small cell lung cancer. Thorac Cancer. 2021; 12(5):659-666. PMC: 7919130. DOI: 10.1111/1759-7714.13823. View

Yang G, Wang T, Qu X, Chen S, Han Z, Chen S . Exosomal miR-21/Let-7a ratio distinguishes non-small cell lung cancer from benign pulmonary diseases. Asia Pac J Clin Oncol. 2020; 16(4):280-286. PMC: 7496917. DOI: 10.1111/ajco.13343. View

10.

Kitagawa T, Taniuchi K, Tsuboi M, Sakaguchi M, Kohsaki T, Okabayashi T . Circulating pancreatic cancer exosomal RNAs for detection of pancreatic cancer. Mol Oncol. 2018; 13(2):212-227. PMC: 6360365. DOI: 10.1002/1878-0261.12398. View

11.

Zhang K, Chen J, Li C, Yuan Y, Fang S, Liu W . Exosome-mediated transfer of SNHG7 enhances docetaxel resistance in lung adenocarcinoma. Cancer Lett. 2021; 526:142-154. DOI: 10.1016/j.canlet.2021.10.029. View

12.

Zheng D, Zhang J, Ni J, Luo J, Wang J, Tang L . Small nucleolar RNA 78 promotes the tumorigenesis in non-small cell lung cancer. J Exp Clin Cancer Res. 2015; 34:49. PMC: 4472183. DOI: 10.1186/s13046-015-0170-5. View

13.

Luo Y, Luo L, Wampfler J, Wang Y, Liu D, Chen Y . 5-year overall survival in patients with lung cancer eligible or ineligible for screening according to US Preventive Services Task Force criteria: a prospective, observational cohort study. Lancet Oncol. 2019; 20(8):1098-1108. PMC: 6669095. DOI: 10.1016/S1470-2045(19)30329-8. View

14.

Murillo O, Thistlethwaite W, Rozowsky J, Subramanian S, Lucero R, Shah N . exRNA Atlas Analysis Reveals Distinct Extracellular RNA Cargo Types and Their Carriers Present across Human Biofluids. Cell. 2019; 177(2):463-477.e15. PMC: 6616370. DOI: 10.1016/j.cell.2019.02.018. View

15.

Leiter A, Veluswamy R, Wisnivesky J . The global burden of lung cancer: current status and future trends. Nat Rev Clin Oncol. 2023; 20(9):624-639. DOI: 10.1038/s41571-023-00798-3. View

16.

Wang X, Tian L, Lu J, Ng I . Exosomes and cancer - Diagnostic and prognostic biomarkers and therapeutic vehicle. Oncogenesis. 2022; 11(1):54. PMC: 9477829. DOI: 10.1038/s41389-022-00431-5. View

17.

Gao L, Ma J, Mannoor K, Guarnera M, Shetty A, Zhan M . Genome-wide small nucleolar RNA expression analysis of lung cancer by next-generation deep sequencing. Int J Cancer. 2014; 136(6):E623-9. DOI: 10.1002/ijc.29169. View

18.

Sung H, Ferlay J, Siegel R, Laversanne M, Soerjomataram I, Jemal A . Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin. 2021; 71(3):209-249. DOI: 10.3322/caac.21660. View

19.

Lv C, Ding W, Wang Y, Zhao Z, Li J, Chen Y . A PEG-based method for the isolation of urinary exosomes and its application in renal fibrosis diagnostics using cargo miR-29c and miR-21 analysis. Int Urol Nephrol. 2018; 50(5):973-982. DOI: 10.1007/s11255-017-1779-4. View

20.

Li S, Qi Y, Huang Y, Guo Y, Huang T, Jia L . Exosome-derived SNHG16 sponging miR-4500 activates HUVEC angiogenesis by targeting GALNT1 via PI3K/Akt/mTOR pathway in hepatocellular carcinoma. J Physiol Biochem. 2021; 77(4):667-682. DOI: 10.1007/s13105-021-00833-w. View