Effects of Alkaline Salt Stress on Growth, Physiological Properties and Medicinal Components of Clonal Glechoma Longituba (Nakai) Kupr

Overview

Journal BMC Plant Biol

Publisher Biomed Central

Specialty Biology

Date 2024 Oct 14

PMID 39402458

Authors

Donghai Wang

Fangshuai Song

Yitong Zhou

Tingting Zhong

Yuyan Zhang

Qiao Deng

Xinqi Wang

Siqi Wang

Daocai Wang

Xiqiang Zhu

Ning Jiang

Xiaopeng Liu

Affiliations

Soon will be listed here.

Abstract

Background: Glechoma longituba, recognized as a medicinal plant, provides valuable pharmaceutical raw materials for treating various diseases. Saline-alkali stress may effectively enhance the medicinal quality of G. longituba by promoting the synthesis of secondary metabolites. To investigate the changes in the primary medicinal components of G. longituba under saline-alkali stress and improve the quality of medicinal materials, NaCO was applied to induce short-term stress under different conditions and the biomass, physiologically active substances and primary medicinal components of G. longituba were measured in this study.

Results: Under alkaline salt stress, the activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), and ascorbate peroxidase (APX) were elevated in G. longituba, accompanied by increased accumulation of proline (Pro) and malondialdehyde (MDA). Furthermore, analysis of the medicinal constituents revealed that G. longituba produced the highest levels of soluble sugars, flavonoids, ursolic acid, and oleanolic acid under 0.6% NaCO stress for 48 h, 0.2% NaCO stress for 72 h, 0.4% NaCO stress for 12 h, and 0.4% NaCO stress for 8 h, respectively.

Conclusions: Short-term NaCO stress enhances the synthesis of medicinal components in G. longituba. By manipulating stress conditions, the production of various medicinal substances could be optimized. This approach may serve as a basis for the targeted cultivation of G. longituba, offering potential applications in the treatment of diverse diseases.

References

Hasanuzzaman M, Bhuyan M, Zulfiqar F, Raza A, Mohsin S, Mahmud J . Reactive Oxygen Species and Antioxidant Defense in Plants under Abiotic Stress: Revisiting the Crucial Role of a Universal Defense Regulator. Antioxidants (Basel). 2020; 9(8). PMC: 7465626. DOI: 10.3390/antiox9080681. View

Zhu Y, Zou J, Zhao W . Two new monoterpenoid glycosides from Glechoma longituba. J Asian Nat Prod Res. 2008; 10(1-2):199-204. DOI: 10.1080/10286020701394480. View

Chen J, Liu Y, Zhang T, Zhou Z, Huang J, Zhou T . Integrated physiological and transcriptional dissection reveals the core genes involving nutrient transport and osmoregulatory substance biosynthesis in allohexaploid wheat seedlings under salt stress. BMC Plant Biol. 2022; 22(1):502. PMC: 9608917. DOI: 10.1186/s12870-022-03887-0. View

Xie L, Lehvavirta S, Timonen S, Kasurinen J, Niemikapee J, Valkonen J . Species-specific synergistic effects of two plant growth-promoting microbes on green roof plant biomass and photosynthetic efficiency. PLoS One. 2019; 13(12):e0209432. PMC: 6312232. DOI: 10.1371/journal.pone.0209432. View

Zhang Y, Fernie A . On the role of the tricarboxylic acid cycle in plant productivity. J Integr Plant Biol. 2018; 60(12):1199-1216. DOI: 10.1111/jipb.12690. View

Isayenkov S, Hilo A, Rizzo P, Tandron Moya Y, Rolletschek H, Borisjuk L . Adaptation Strategies of Halophytic Barley ssp. to High Salinity and Osmotic Stress. Int J Mol Sci. 2020; 21(23). PMC: 7730945. DOI: 10.3390/ijms21239019. View

Bai Q, Niu Z, Chen Q, Gao C, Zhu M, Bai J . The C H -type zinc finger transcription factor OSIC1 positively regulates stomatal closure under osmotic stress in poplar. Plant Biotechnol J. 2023; 21(5):943-960. PMC: 10106854. DOI: 10.1111/pbi.14007. View

Agati G, Brunetti C, Fini A, Gori A, Guidi L, Landi M . Are Flavonoids Effective Antioxidants in Plants? Twenty Years of Our Investigation. Antioxidants (Basel). 2020; 9(11). PMC: 7695271. DOI: 10.3390/antiox9111098. View

Ullah A, Tariq A, Sardans J, Penuelas J, Zeng F, Graciano C . Alhagi sparsifolia acclimatizes to saline stress by regulating its osmotic, antioxidant, and nitrogen assimilation potential. BMC Plant Biol. 2022; 22(1):453. PMC: 9490911. DOI: 10.1186/s12870-022-03832-1. View

10.

Zhang D, Tong J, He X, Xu Z, Xu L, Wei P . A Novel Soybean Intrinsic Protein Gene, GmTIP2;3, Involved in Responding to Osmotic Stress. Front Plant Sci. 2016; 6:1237. PMC: 4705450. DOI: 10.3389/fpls.2015.01237. View

11.

Zhang M, Yu Z, Zeng D, Si C, Zhao C, Wang H . Transcriptome and Metabolome Reveal Salt-Stress Responses of Leaf Tissues from . Biomolecules. 2021; 11(5). PMC: 8156352. DOI: 10.3390/biom11050736. View

12.

Zhou R, Huang J, He D, Yi Z, Zhao D, Liu Z . Green and Efficient Extraction of Polysaccharide and Ginsenoside from American Ginseng ( L.) by Deep Eutectic Solvent Extraction and Aqueous Two-Phase System. Molecules. 2022; 27(10). PMC: 9144842. DOI: 10.3390/molecules27103132. View

13.

Shomali A, Das S, Arif N, Sarraf M, Zahra N, Yadav V . Diverse Physiological Roles of Flavonoids in Plant Environmental Stress Responses and Tolerance. Plants (Basel). 2022; 11(22). PMC: 9699315. DOI: 10.3390/plants11223158. View

14.

Wang C, Chen L, Cai Z, Chen C, Liu Z, Liu S . Metabolite Profiling and Transcriptome Analysis Explains Difference in Accumulation of Bioactive Constituents in Licorice () Under Salt Stress. Front Plant Sci. 2021; 12:727882. PMC: 8529186. DOI: 10.3389/fpls.2021.727882. View

15.

Sies H, Jones D . Reactive oxygen species (ROS) as pleiotropic physiological signalling agents. Nat Rev Mol Cell Biol. 2020; 21(7):363-383. DOI: 10.1038/s41580-020-0230-3. View

16.

Wozniak L, Skapska S, Marszalek K . Ursolic Acid--A Pentacyclic Triterpenoid with a Wide Spectrum of Pharmacological Activities. Molecules. 2015; 20(11):20614-41. PMC: 6332387. DOI: 10.3390/molecules201119721. View

17.

Park C, Lim C, Lee S . The Pepper CaOSR1 Protein Regulates the Osmotic Stress Response via Abscisic Acid Signaling. Front Plant Sci. 2016; 7:890. PMC: 4919342. DOI: 10.3389/fpls.2016.00890. View

18.

Mitra S, Lami M, Uddin T, Das R, Islam F, Anjum J . Prospective multifunctional roles and pharmacological potential of dietary flavonoid narirutin. Biomed Pharmacother. 2022; 150:112932. DOI: 10.1016/j.biopha.2022.112932. View

19.

Yang J, Zhao S, Zhao B, Li C . Overexpression of TaLEA3 induces rapid stomatal closure under drought stress in Phellodendron amurense Rupr. Plant Sci. 2018; 277:100-109. DOI: 10.1016/j.plantsci.2018.09.022. View

20.

Yang Y, Yao Y, Li J, Zhang J, Zhang X, Hu L . Trehalose Alleviated Salt Stress in Tomato by Regulating ROS Metabolism, Photosynthesis, Osmolyte Synthesis, and Trehalose Metabolic Pathways. Front Plant Sci. 2022; 13:772948. PMC: 8963455. DOI: 10.3389/fpls.2022.772948. View