Transcriptional Landscape of Sweetpotato Root Tip Development at the Single-cell Level

Overview

Journal BMC Plant Biol

Publisher Biomed Central

Specialty Biology

Date 2024 Oct 11

PMID 39394068

Authors

Nan Zhao

Xiawei Ding

Caihuan Tian

Shixin Wang

Shuyan Xie

Hongda Zou

Hao Liu

Jingyi Chen

Xue Lian Liang

Lifei Huang

Affiliations

Soon will be listed here.

Abstract

Single-cell transcriptome sequencing (scRNA-seq) is a powerful tool for describing the transcriptome dynamics of plant development but has not yet been utilized to analyze the tissue ontology of sweetpotato. This study established a stable method for isolating single protoplast cells for scRNA-seq to reveal the cell heterogeneity of sweetpotato root tip meristems at the single-cell level. The study analyzed 12,172 single cells and 27,355 genes in the root tips of the sweetpotato variety Guangshu 87, which were distributed into 15 cell clusters. Pseudo-time analysis showed that there were transitional cells in the apical development trajectory of mature cell types from stem cell niches. Furthermore, we identified novel development regulators of sweetpotato tubers via trajectory analysis. The transcription factor IbGATA4 was highly expressed in the adventitious roots during the development of sweetpotato root tips, where it may regulate the development of sweetpotato root tips. In addition, significant differences were observed in the transcriptional profiles of cell types between sweetpotato, Arabidopsis thaliana, and maize. This study mapped the single-cell transcriptome of sweetpotato root tips, laying a foundation for studying the types, functions, differentiation, and development of sweetpotato root tip cells.

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