LasR-deficient Mutants Have Increased Methylglyoxal and Hydrogen Peroxide Sensitivity Due to Low Intracellular Glutathione

The electrophile methylglyoxal (MG) is produced by microorganisms and host cells through central metabolic pathways. MG is a highly reactive electrophile, so it must be rapidly detoxified to prevent damaging modifications to macromolecules. , a pathogen of concern due to its ability develop multidrug resistance, causes many types of infections that have been associated with elevated MG levels, including cystic fibrosis (CF). isolates commonly have mutations that lead to LasR loss-of-function (LasR-) and we found that mutations confer sensitivity to MG in multiple strain backgrounds. LasR- strains have increased activity of the CbrAB two-component system which represses Crc regulation of metabolism. Here, we show that higher CbrAB activity and low Crc activity renders cells sensitive to MG. We found that LasR- strains are more sensitive to MG and have lower intracellular reduced glutathione (GSH) compared to their LasR+ comparators. Consistent with published reports, mutants lacking , which encodes a MG-glyoxalase, and mutants lacking GSH biosynthesis enzymes ( or ) were sensitive to MG. Exogenous GSH rescued MG sensitivity in LasR- strains and or mutants, but not in a mutant strain. We propose that low GSH levels in LasR- strains contribute to increased sensitivity to MG and HO.