Effect of a Single Dose of Letrozole on Ejaculation Time, Semen Quality, and Testicular Hemodynamics in Goat Bucks Subjected to Heat Stress

Overview

Journal Vet Res Commun

Publisher Springer

Specialty Veterinary Medicine

Date 2024 Oct 9

PMID 39382810

Authors

Ola Adel

Hossam R El-Sherbiny

Abdallah M Shahat

Sayed Taha Ismail

Affiliations

Soon will be listed here.

Abstract

Letrozole (LTZ) is an aromatase inhibitor that limits estrogen (E2) production and increases testosterone (T) levels. This research aimed to examine the impact of a single dose of LTZ on testicular hemodynamics, ejaculation time, and semen quality in goats under heat stress (HS). Therefore, Doppler examination and semen evaluation were performed on twelve mature bucks for two weeks (W-1, W-2) as pre-heat stress control during winter. Then during summer HS bucks were subjected to Doppler examination, semen evaluation, and hormonal analysis (T, E2, and LH) at 0 h. Afterward, bucks were assigned into two groups and subcutaneously injected with physiological saline (n = 6; CON) or LTZ (0.25 mg/kg BW; n = 6; LTZ). Both groups were subjected to Doppler scanning and hormonal analysis at 2, 4, 24, 48, 72, 96,144, and 168 h. Semen evaluation was performed at 48 and 168 h. The LTZ group showed increasing (P < 0.05) in semen volume, sperm motility, and viability and decreasing (P < 0.05) in ejaculation time and sperm abnormalities compared to CON group at 48 h. Additionally, T concentrations increased (P < 0.01) at 2, 24, and 48 h, E2 decreased (P < 0.01) from 2 to 48 h, and LH raised (P < 0.01) at 2 and 72 h in LTZ group compared to CON one. Doppler indices reduced (P < 0.05) at 96 h in LTZ group. semen pH and scrotal circumference were not affected by LTZ. In conclusion, LTZ administration shortened ejaculation time and enhanced semen quality in bucks during HS.

References

Esteller-Vico A, Ball B, Troedsson M, Squires E . Endocrine changes, fetal growth, and uterine artery hemodynamics after chronic estrogen suppression during the last trimester of equine pregnancy. Biol Reprod. 2017; 96(2):414-423. DOI: 10.1095/biolreprod.116.140533. View

Mukherjee A, Wanjari U, Nagarajan D, K K V, V A, P J . Letrozole: Pharmacology, toxicity and potential therapeutic effects. Life Sci. 2022; 310:121074. DOI: 10.1016/j.lfs.2022.121074. View

Kivrak M, Corum O, Alkan H, Atik O, Aydin I, Uney K . The pharmacokinetics of letrozole and its effect on gonadotropins in anestrous ewes. Theriogenology. 2021; 176:225-232. DOI: 10.1016/j.theriogenology.2021.09.033. View

At-Taras E, Conley A, Berger T, Roser J . Reducing estrogen synthesis does not affect gonadotropin secretion in the developing boar. Biol Reprod. 2005; 74(1):58-66. DOI: 10.1095/biolreprod.105.043760. View

Pragna P, Sejian V, Bagath M, Krishnan G, Archana P, Soren N . Comparative assessment of growth performance of three different indigenous goat breeds exposed to summer heat stress. J Anim Physiol Anim Nutr (Berl). 2018; 102(4):825-836. DOI: 10.1111/jpn.12892. View

Li L, Lin W, Wang Z, Huang R, Xia H, Li Z . Hormone Regulation in Testicular Development and Function. Int J Mol Sci. 2024; 25(11). PMC: 11172568. DOI: 10.3390/ijms25115805. View

Nowicka-Bauer K, Nixon B . Molecular Changes Induced by Oxidative Stress that Impair Human Sperm Motility. Antioxidants (Basel). 2020; 9(2). PMC: 7070831. DOI: 10.3390/antiox9020134. View

El-Sherbiny H, Fathi M, Samir H, Abdelnaby E . Supplemental dietary curcumin improves testicular hemodynamics, testosterone levels, and semen quality in Baladi bucks in the non-breeding season. Theriogenology. 2022; 188:100-107. DOI: 10.1016/j.theriogenology.2022.05.020. View

Amaral A . Energy metabolism in mammalian sperm motility. WIREs Mech Dis. 2022; 14(5):e1569. DOI: 10.1002/wsbm.1569. View

10.

Garzon S, Lagana A, Barra F, Casarin J, Cromi A, Raffaelli R . Aromatase inhibitors for the treatment of endometriosis: a systematic review about efficacy, safety and early clinical development. Expert Opin Investig Drugs. 2020; 29(12):1377-1388. DOI: 10.1080/13543784.2020.1842356. View

11.

Adamczewska D, Slowikowska-Hilczer J, Walczak-Jedrzejowska R . The Fate of Leydig Cells in Men with Spermatogenic Failure. Life (Basel). 2022; 12(4). PMC: 9028943. DOI: 10.3390/life12040570. View

12.

Naelitz B, Jiang T, Munoz-Lopez C, Sigalos J, Modiri N, Cannarella R . Testosterone and luteinizing hormone predict semen parameter improvement in infertile men treated with anastrozole. Fertil Steril. 2023; 120(4):746-754. DOI: 10.1016/j.fertnstert.2023.06.032. View

13.

El-Sherbiny H, El-Shalofy A, Samir H . Exogenous L-carnitine Administration Ameliorates the Adverse Effects of Heat Stress on Testicular Hemodynamics, Echotexture, and Total Antioxidant Capacity in Rams. Front Vet Sci. 2022; 9:860771. PMC: 9019560. DOI: 10.3389/fvets.2022.860771. View

14.

Rezaei A, Vaziry A, Farshad A, Farzinpour A, Rostamzadeh J . Effects of letrozole administration on growth and reproductive performance in Markhoz goat bucklings. Theriogenology. 2019; 147:183-191. DOI: 10.1016/j.theriogenology.2019.11.017. View

15.

Oduwole O, Huhtaniemi I, Misrahi M . The Roles of Luteinizing Hormone, Follicle-Stimulating Hormone and Testosterone in Spermatogenesis and Folliculogenesis Revisited. Int J Mol Sci. 2021; 22(23). PMC: 8658012. DOI: 10.3390/ijms222312735. View

16.

Giriboni J, Gokdal O, Eren V, Yarali E, Santiago-Moreno J, Ungerfeld R . Daily administration of a GnRH analogue enhances sperm quality in bucks during the non-breeding season. Anim Reprod Sci. 2018; 200:43-50. DOI: 10.1016/j.anireprosci.2018.11.009. View

17.

El-Shalofy A, Samir H, El-Sherbiny H . Intramuscular administration of l-arginine boosts testicular hemodynamics, plasma concentrations of testosterone and nitric oxide in heat-stressed rams. Theriogenology. 2022; 197:127-132. DOI: 10.1016/j.theriogenology.2022.11.030. View

18.

Yan R, Yang Q, Yang Q . E4 Transcription Factor 1 (E4F1) Regulates Sertoli Cell Proliferation and Fertility in Mice. Animals (Basel). 2020; 10(9). PMC: 7552733. DOI: 10.3390/ani10091691. View

19.

Kim M, Cha K, Hwang S, Park U, Seo S, Lee S . Pectinase-treated protects heat stress-induced testicular damage in rats. Reproduction. 2017; 153(6):737-747. DOI: 10.1530/REP-16-0560. View

20.

Ali E, Zhandi M, Towhidi A, Zaghari M, Ansari M, Najafi M . Letrozole, an aromatase inhibitor, reduces post-peak age-related regression of rooster reproductive performance. Anim Reprod Sci. 2017; 183:110-117. DOI: 10.1016/j.anireprosci.2017.05.010. View