Multi-scale Phenotyping of Senescence-related Changes in Roots of Rapeseed in Response to Nitrate Limitation

Overview

Journal J Exp Bot

Publisher Oxford University Press

Specialty Biology

Date 2024 Oct 9

PMID 39382543

Authors

Maxence James

Celine Masclaux-Daubresse

Thierry Balliau

Anne Marmagne

Fabien Chardon

Jacques Trouverie

Philippe Etienne

Affiliations

Soon will be listed here.

Abstract

Root senescence remains largely unexplored. In this study, the time-course of the morphological, metabolic, and proteomic changes occurring with root aging were investigated, providing a comprehensive picture of the root senescence program. We found novel senescence-related markers for the characterization of the developmental stage of root tissues. The rapeseed root system is unique in that it consists of the taproot and lateral roots. Our study confirmed that the taproot, which transiently accumulates large quantities of starch and proteins, is specifically dedicated to nutrient storage and remobilization, while the lateral roots are mainly dedicated to nutrient uptake. Proteomic data from the taproot and lateral roots highlighted the different senescence-related events that control nutrient remobilization and nutrient uptake capacities. Both the proteome and enzyme activities revealed senescence-induced proteases and nucleotide catabolic enzymes that deserve attention as they may play important roles in nutrient remobilization efficiency in rapeseed roots. Taking advantage of publicly available transcriptomic and proteomic data on senescent Arabidopsis leaves, we provide a novel lists of senescence-related proteins specific or common to root organs and/or leaves.

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