Assessment of the Effects of Albendazole-Loaded Sulfonated Graphene Oxide on Protoscoleces: An Investigation

Overview

Journal J Trop Med

Publisher Wiley

Specialty Tropical Medicine

Date 2024 Oct 7

PMID 39372238

Authors

Mohammad Reza Lashkarizadeh

Mohammad Shafieei

Mahdiyeh Lashkarizadeh

Seyed Mohammad Mousavi

Ghazaleh Sheibani

Zahra Akbari

Haniyeh Daneshafruz

Ali Derakhshani

Faham Khamesipour

Affiliations

Soon will be listed here.

Abstract

Objectives: Due to Albendazole's relatively low efficacy and bioavailability, Echinococcosis has proven a challenge to manage successfully, with several studies investigating ways to improve the outcome, mainly showing mixed results. We, therefore, aimed to evaluate whether Sulfonated Graphene Oxide (S-GO), as nanocarriers, could improve the mentioned outcome.

Methods: protoscoleces were divided into four groups based on the agent they received, which comprised control, S-GO, Albendazole, and Albendazole-loaded S-GO (S-GO-Albendazole). Then, the and gene expression levels and the number of surviving protoscoleces in each group were determined.

Results: gene expression increased by 121% in the 50 g/ml concentration of the S-GO-Albendazole, while gene expression decreased by 64%. Moreover, S-GO-Albendazole was approximately 18% more effective at neutralizing protoscoleces than Albendazole and 14% and 31% more effective at improving the expression of the mentioned genes, respectively ( < 0.05). In addition, the number of surviving protoscoleces after exposure to the mentioned concentration reduced by approximately 99%.

Conclusions: S-GO, despite not having significant lethality on protoscoleces, significantly increased the lethality of Albendazole and, therefore, is a suitable nanocarrier. However, we recommend conducting and clinical studies to more accurately determine this nanocomplex's potential and side effects.

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