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Charcoal-yeast Extract Agar: Primary Isolation Medium for Legionella Pneumophila

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 1979 Oct 1
PMID 393713
Citations 301
Authors
J C Feeley
R J Gibson
G W GORMAN
N C Langford
J K Rasheed
D C Mackel
W B Baine
Affiliations
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Abstract

Charcoal-yeast extract agar is a new bacteriological medium that supports excellent growth of the Legionella pneumophila. It results from modifications made in an existing L. pneumophila medium, F-G agar. Yeast extract, instead of an acid hydrolysate of casein, serves as the protein source. Beef extractives and starch are not added. Activated charcoal (Norit A or Norit SG) is included at 0.20% (wt/vol). Comparison of charcoal-yeast extract and F-G agars showed that a greater number of colony-forming units of L. pneumophila was recovered from a standardized tissue inoculum on charcoal-yeast extract agar (4.35 x 10(6) colony-forming units) than on F-G agar (4.85 x 10(4) colony-forming units). Macroscopic colonies of L. pneumophila were visible on the new medium within 3 days, whereas 4 days of growth was required on F-G agar.

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References
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