Bactericidal, Anti-hemolytic, and Anticancerous Activities of Phytofabricated Silver Nanoparticles of Glycine Max Seeds

Overview

Journal Front Chem

Specialty Chemistry

Date 2024 Oct 4

PMID 39364440

Authors

K B Vijendra Kumar

Kavitha Raj Varadaraju

Prasanna D Shivaramu

C M Hemanth Kumar

H R Prakruthi

B M Chandra Shekara

Bhargav Shreevatsa

Tanveer A Wani

K C Prakasha

Shiva Prasad Kollur

Chandan Shivamallu

Affiliations

Soon will be listed here.

Abstract

Introduction: Soybean is a rich source of bioactive components with good nutritional support and is easily available. In the treatment of cancer, green synthesis of silver nanoparticles (AgNPs) from plant-based samples has gained attentions due to its potency and feasibility. In the present study, using soybean extracts (GM), silver nanoparticles are synthesized and analyzed for their anticancer potency.

Methods: The synthesized GM-AgNPs were characterized via UV-Vis spectroscopy, Fourier transform-infrared (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy-dispersive X-ray (EDX) techniques for further analysis. Antibacterial activity was evaluated using the disc method and anti-hemolysis activity using the method, followed by anticancer property evaluation by cytotoxicity, cell migration, apoptosis, and cell cycle.

Results And Discussion: Our results showed that the synthesized GM-AgNPs were spiral-shaped with a size range of 5-50 nm. The antibacterial activity against and showed the maximum zone of inhibition at 250 μg/mL in comparison with gentamicin. On exploring the anti-hemolysis efficiency, at 200 μg/mL, GM-AgNPs showed no hemolysis in comparison to the extract which showed 40% hemolysis. On analysis of GM-AgNPs against the breast cancer cell line, the nanoparticles displayed the IC50 value of 74.04 μg/mL. Furthermore, at the IC50 concentration, cancer cell migration was reduced. The mechanism of action of GM-AgNPs confirmed the initiation of apoptosis and cell cycle arrest in the sub-G0/G1 (growth phase) phase by 48.19%. In gene expression and protein expression analyses, Bax and Bcl-2 were altered to those of normal physiology.

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