The Effect and Mechanism of Freeze-dried Powder of on Improving Inflammatory Injury of Rat Glomerular Mesangial Cells Through TXNIP / NLRP3 Pathway

Overview

Journal Heliyon

Specialty Social Sciences

Date 2024 Oct 4

PMID 39364251

Authors

Xi Sun

Maiheliya Mijiti

Chuyin Huang

Shanshan Mei

Kexin Fang

Yaojun Yang

Affiliations

Soon will be listed here.

Abstract

Objective: Diabetic kidney disease (DKD) is a common complication of . The pathophysiological changes in platelet function and the hypercoagulable state associated with DKD are closely linked to inflammatory processes. (PM), a type of leech known for its anticoagulant and antithrombotic properties, has the potential to modulate the inflammatory response in DKD. This study aims to investigate the effect of freeze-dried powder of PM on improving inflammatory injury in rat glomerular mesangial cells and to explore its underlying mechanism.

Methods: Lipopolysaccharide (LPS) stimulated HBZY-1 rat mesangial cells to establish an DKD inflammation model. After the intervention with the water extract of freeze-dried powder of PM (FDPM), cell viability, NO content, and the levels of inflammatory factors such as IL-1β, IL-18, and TNF-α were assessed. Finally, utilizing transcriptomics technology, RT-qPCR, and Western blot methods, the mechanism by which FDPM improves inflammatory injury in rat glomerular mesangial cells was explored and preliminarily validated.

Results: FDPM effectively enhances cell viability and inhibits the production of NO and related inflammatory factors. Transcriptomic analysis suggests that FDPM may exert these effects by regulating the TXNIP/NLRP3 signaling pathway. The mRNA and protein expressions of TXNIP, NLRP3, and MCP-1 in the model cells were reversed by FDPM.

Conclusion: FDPM may improve the micro-inflammatory state of DKD and slow the progression of the disease by regulating the TXNIP/NLRP3 signaling pathway. This study provides a scientific basis for the clinical application of PM DKD treatment.

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