A HD-ZIP Gene is Involved in Modulation of Root Development by Regulating Auxin Response

Overview

Journal Front Plant Sci

Date 2024 Oct 4

PMID 39363924

Authors

Wei Yan

Runze Wang

Yutong Zhang

Xiuxiu Zhang

Qin Wang

Affiliations

Soon will be listed here.

Abstract

HD-Zip proteins are plant-specific transcription factors known for their diverse functions in regulating plant growth, development, and responses to environmental stresses. Among the HD-Zip II genes, has been previously linked to abiotic stress responses. In this study, we conducted a functional characterization of in the regulation of root growth and development. Upon auxin stimulation, expression of was promptly up-regulated. Overexpression of in led to reduced primary root growth and inhibited lateral root formation. Interestingly, the transgenic plants expressing exhibited differential responses to three types of auxins (IAA, NAA, and 2,4-D) in terms of root growth and development compared to the wild-type plants. Specifically, primary root growth was less affected, and lateral root formation was enhanced in the transgenic plants when exposed to auxins. This differential response suggests a potential role for in modulating auxin transport and accumulation, as evidenced by the reduced sensitivity of the transgenic plants to the auxin transport inhibitor NPA and lower expression levels of auxin-related reporters such as - ()::PIN1-GFP, ::PIN3-GFP, ::PIN7-GFP, and ::GFP compared to wild-type plants. Additionally, microarray analysis of the root tissues revealed down-regulation of several auxin-responsive genes in transgenic seedlings compared to wild-type plants. These findings collectively indicate that plays a critical regulatory role in root growth and development by modulating auxin accumulation and response in the roots.

References

Whipple C, Kebrom T, Weber A, Yang F, Hall D, Meeley R . grassy tillers1 promotes apical dominance in maize and responds to shade signals in the grasses. Proc Natl Acad Sci U S A. 2011; 108(33):E506-12. PMC: 3158142. DOI: 10.1073/pnas.1102819108. View

Brandt R, Cabedo M, Xie Y, Wenkel S . Homeodomain leucine-zipper proteins and their role in synchronizing growth and development with the environment. J Integr Plant Biol. 2014; 56(6):518-26. DOI: 10.1111/jipb.12185. View

Kazan K, Manners J . JAZ repressors and the orchestration of phytohormone crosstalk. Trends Plant Sci. 2011; 17(1):22-31. DOI: 10.1016/j.tplants.2011.10.006. View

Kollmer I, Werner T, Schmulling T . Ectopic expression of different cytokinin-regulated transcription factor genes of Arabidopsis thaliana alters plant growth and development. J Plant Physiol. 2011; 168(12):1320-7. DOI: 10.1016/j.jplph.2011.02.006. View

Ni J, Shen Y, Zhang Y, Liu Y . Histological characterization of the lateral root primordium development in rice. Bot Stud. 2017; 55(1):42. PMC: 5432738. DOI: 10.1186/s40529-014-0042-x. View

Benkova E, Michniewicz M, Sauer M, Teichmann T, Seifertova D, Jurgens G . Local, efflux-dependent auxin gradients as a common module for plant organ formation. Cell. 2003; 115(5):591-602. DOI: 10.1016/s0092-8674(03)00924-3. View

Paponov I, Paponov M, Teale W, Menges M, Chakrabortee S, Murray J . Comprehensive transcriptome analysis of auxin responses in Arabidopsis. Mol Plant. 2009; 1(2):321-37. DOI: 10.1093/mp/ssm021. View

Ariel F, Manavella P, Dezar C, Chan R . The true story of the HD-Zip family. Trends Plant Sci. 2007; 12(9):419-26. DOI: 10.1016/j.tplants.2007.08.003. View

Casimiro I, Beeckman T, Graham N, Bhalerao R, Zhang H, Casero P . Dissecting Arabidopsis lateral root development. Trends Plant Sci. 2003; 8(4):165-71. DOI: 10.1016/S1360-1385(03)00051-7. View

10.

Gong S, Ding Y, Hu S, Ding L, Chen Z, Zhu C . The role of HD-Zip class I transcription factors in plant response to abiotic stresses. Physiol Plant. 2019; 167(4):516-525. DOI: 10.1111/ppl.12965. View

11.

Harris J, Hrmova M, Lopato S, Langridge P . Modulation of plant growth by HD-Zip class I and II transcription factors in response to environmental stimuli. New Phytol. 2011; 190(4):823-837. DOI: 10.1111/j.1469-8137.2011.03733.x. View

12.

Sawa S, Ohgishi M, Goda H, Higuchi K, Shimada Y, Yoshida S . The HAT2 gene, a member of the HD-Zip gene family, isolated as an auxin inducible gene by DNA microarray screening, affects auxin response in Arabidopsis. Plant J. 2002; 32(6):1011-22. DOI: 10.1046/j.1365-313x.2002.01488.x. View

13.

Gu Z, Zhou X, Li S, Pang Y, Xu Y, Zhang X . The HD-ZIP IV transcription factor GLABRA2 acts as an activator for proanthocyanidin biosynthesis in Medicago truncatula seed coat. Plant J. 2024; 119(5):2303-2315. DOI: 10.1111/tpj.16918. View

14.

Clough S, Bent A . Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1999; 16(6):735-43. DOI: 10.1046/j.1365-313x.1998.00343.x. View

15.

Li Y, Han S, Qi Y . Advances in structure and function of auxin response factor in plants. J Integr Plant Biol. 2022; 65(3):617-632. DOI: 10.1111/jipb.13392. View

16.

Steindler C, Matteucci A, Sessa G, Weimar T, Ohgishi M, Aoyama T . Shade avoidance responses are mediated by the ATHB-2 HD-zip protein, a negative regulator of gene expression. Development. 1999; 126(19):4235-45. DOI: 10.1242/dev.126.19.4235. View

17.

Peret B, De Rybel B, Casimiro I, Benkova E, Swarup R, Laplaze L . Arabidopsis lateral root development: an emerging story. Trends Plant Sci. 2009; 14(7):399-408. DOI: 10.1016/j.tplants.2009.05.002. View

18.

Johnson J, Vance C, Allan D . Phosphorus deficiency in Lupinus albus. Altered lateral root development and enhanced expression of phosphoenolpyruvate carboxylase. Plant Physiol. 1996; 112(1):31-41. PMC: 157920. DOI: 10.1104/pp.112.1.31. View

19.

Lee H, Kim H, Park J, Cho H, Jeon J . PIN-mediated polar auxin transport facilitates root-obstacle avoidance. New Phytol. 2019; 225(3):1285-1296. DOI: 10.1111/nph.16076. View

20.

He G, Liu P, Zhao H, Sun J . The HD-ZIP II Transcription Factors Regulate Plant Architecture through the Auxin Pathway. Int J Mol Sci. 2020; 21(9). PMC: 7246542. DOI: 10.3390/ijms21093250. View