Proteomic Analysis of Plasma-derived Extracellular Vesicles: Pre- and Postprandial Comparisons

Overview

Journal Sci Rep

Specialty Science

Date 2024 Oct 3

PMID 39363010

Authors

Alejandra P Garza

Elisa Wider-Eberspacher

Lorena Morton

Marco van Ham

Eva Pallinger

Edit I Buzas

Lothar Jansch

Ildiko R Dunay

Affiliations

Soon will be listed here.

Abstract

Extracellular vesicles (EVs) are key in intercellular communication, carrying biomolecules like nucleic acids, lipids, and proteins. This study investigated postprandial characteristics and proteomic profiles of blood-derived EVs in healthy individuals. Twelve participants fasted overnight before baseline assessments. After consuming a controlled isocaloric meal, EVs were isolated for proteomic and flow cytometric analysis. Plasma triacylglyceride levels confirmed fasting completion, while protein concentrations in plasma and EVs were monitored for postprandial stability. Proteomic analysis identified upregulated proteins related to transport mechanisms and epithelial/endothelial functions postprandially, indicating potential roles in physiological responses to nutritional intake. Enrichment analyses revealed vesicle-related pathways and immune system processes. Flow cytometry showed increased expression of CD324 on CD9CD63CD81 large extracellular vesicles postprandially, suggesting an epithelial origin. These findings offer valuable insights into postprandial EV dynamics and their potential physiological significance, highlighting the need for stringent fasting guidelines in EV studies to account for postprandial effects on EV composition and function.

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References

Staufer O, Hernandez Bucher J, Fichtler J, Schroter M, Platzman I, Spatz J . Vesicle Induced Receptor Sequestration: Mechanisms behind Extracellular Vesicle-Based Protein Signaling. Adv Sci (Weinh). 2022; 9(13):e2200201. PMC: 9069182. DOI: 10.1002/advs.202200201. View

Chang X, Wang S, Zhao S, Shi Y, Pan P, Gu L . Extracellular Vesicles with Possible Roles in Gut Intestinal Tract Homeostasis and IBD. Mediators Inflamm. 2020; 2020:1945832. PMC: 7201673. DOI: 10.1155/2020/1945832. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Michelsen A, Noto A, Brodin E, Mathiesen E, Brosstad F, Hansen J . Elevated levels of platelet microparticles in carotid atherosclerosis and during the postprandial state. Thromb Res. 2008; 123(6):881-6. DOI: 10.1016/j.thromres.2008.10.016. View

Lasser C, Jang S, Lotvall J . Subpopulations of extracellular vesicles and their therapeutic potential. Mol Aspects Med. 2018; 60:1-14. DOI: 10.1016/j.mam.2018.02.002. View

Salomon C, Das S, Erdbrugger U, Kalluri R, Lim S, Olefsky J . Extracellular Vesicles and Their Emerging Roles as Cellular Messengers in Endocrinology: An Endocrine Society Scientific Statement. Endocr Rev. 2022; 43(3):441-468. PMC: 10686249. DOI: 10.1210/endrev/bnac009. View

Nieuwland R, Siljander P . A beginner's guide to study extracellular vesicles in human blood plasma and serum. J Extracell Vesicles. 2024; 13(1):e12400. PMC: 10775135. DOI: 10.1002/jev2.12400. View

Dong L, Zieren R, Horie K, Kim C, Mallick E, Jing Y . Comprehensive evaluation of methods for small extracellular vesicles separation from human plasma, urine and cell culture medium. J Extracell Vesicles. 2021; 10(2):e12044. PMC: 7810129. DOI: 10.1002/jev2.12044. View

Dixson A, Dawson T, Di Vizio D, Weaver A . Context-specific regulation of extracellular vesicle biogenesis and cargo selection. Nat Rev Mol Cell Biol. 2023; 24(7):454-476. PMC: 10330318. DOI: 10.1038/s41580-023-00576-0. View

10.

Niessen P, Rensen S, van Deursen J, De Man J, De Laet A, Vanderwinden J . Smoothelin-a is essential for functional intestinal smooth muscle contractility in mice. Gastroenterology. 2005; 129(5):1592-601. DOI: 10.1053/j.gastro.2005.08.018. View

11.

Garza A, Morton L, Pallinger E, Buzas E, Schreiber S, Schott B . Initial and ongoing tobacco smoking elicits vascular damage and distinct inflammatory response linked to neurodegeneration. Brain Behav Immun Health. 2023; 28:100597. PMC: 9931921. DOI: 10.1016/j.bbih.2023.100597. View

12.

Qian F, Huang Z, Zhong H, Lei Q, Ai Y, Xie Z . Analysis and Biomedical Applications of Functional Cargo in Extracellular Vesicles. ACS Nano. 2022; 16(12):19980-20001. DOI: 10.1021/acsnano.2c11298. View

13.

Sharma P, Roy A, Dhamija R, Bhushan S, Baswal K, Kulandaisamy R . A comprehensive proteomic profiling of urinary exosomes and the identification of early non-invasive biomarker in patients with coronary artery disease. J Proteomics. 2023; 293:105059. DOI: 10.1016/j.jprot.2023.105059. View

14.

Lyu C, Sun H, Sun Z, Liu Y, Wang Q . Roles of exosomes in immunotherapy for solid cancers. Cell Death Dis. 2024; 15(2):106. PMC: 10834551. DOI: 10.1038/s41419-024-06494-z. View

15.

Yates A, Pink R, Erdbrugger U, Siljander P, Dellar E, Pantazi P . In sickness and in health: The functional role of extracellular vesicles in physiology and pathology in vivo: Part I: Health and Normal Physiology: Part I: Health and Normal Physiology. J Extracell Vesicles. 2022; 11(1):e12151. PMC: 8765331. DOI: 10.1002/jev2.12151. View

16.

Spazierer D, Fuchs P, Proll V, Janda L, Oehler S, Fischer I . Epiplakin gene analysis in mouse reveals a single exon encoding a 725-kDa protein with expression restricted to epithelial tissues. J Biol Chem. 2003; 278(34):31657-66. DOI: 10.1074/jbc.M303055200. View

17.

Sustar V, Bedina-Zavec A, Stukelj R, Frank M, Ogorevc E, Jansa R . Post-prandial rise of microvesicles in peripheral blood of healthy human donors. Lipids Health Dis. 2011; 10:47. PMC: 3071324. DOI: 10.1186/1476-511X-10-47. View

18.

Zhang X, Wang C, Yu J, Bu J, Ai F, Wang Y . Extracellular vesicles in the treatment and diagnosis of breast cancer: a status update. Front Endocrinol (Lausanne). 2023; 14:1202493. PMC: 10393036. DOI: 10.3389/fendo.2023.1202493. View

19.

Ferreira A, Peter A, Mendez A, Jimenez J, Mauro L, Chirinos J . Postprandial hypertriglyceridemia increases circulating levels of endothelial cell microparticles. Circulation. 2004; 110(23):3599-603. DOI: 10.1161/01.CIR.0000148820.55611.6B. View

20.

Scalise A, Kakogiannos N, Zanardi F, Iannelli F, Giannotta M . The blood-brain and gut-vascular barriers: from the perspective of claudins. Tissue Barriers. 2021; 9(3):1926190. PMC: 8489939. DOI: 10.1080/21688370.2021.1926190. View