Cpt1a Drives Primed-to-naïve Pluripotency Transition Through Lipid Remodeling

Overview

Journal Commun Biol

Specialty Biology

Date 2024 Sep 30

PMID 39349670

Authors

Zhaoyi Ma

Xingnan Huang

Junqi Kuang

Qiannan Wang

Yue Qin

Tao Huang

Zechuan Liang

Wei Li

Yu Fu

Pengli Li

Yixin Fan

Ziwei Zhai

Xiaomin Wang

Jin Ming

Chengchen Zhao

Bo Wang

Duanqing Pei

Affiliations

Soon will be listed here.

Abstract

Metabolism has been implicated in cell fate determination, particularly through epigenetic modifications. Similarly, lipid remodeling also plays a role in regulating cell fate. Here, we present comprehensive lipidomics analysis during BMP4-driven primed to naive pluripotency transition or BiPNT and demonstrate that lipid remodeling plays an essential role. We further identify Cpt1a as a rate-limiting factor in BiPNT, driving lipid remodeling and metabolic reprogramming while simultaneously increasing intracellular acetyl-CoA levels and enhancing H3K27ac at chromatin open sites. Perturbation of BiPNT by histone acetylation inhibitors suppresses lipid remodeling and pluripotency transition. Together, our study suggests that lipid remodeling promotes pluripotency transitions and further regulates cell fate decisions, implicating Cpt1a as a critical regulator between primed-naive cell fate control.

Citing Articles

Loss of Cpt1a results in elevated glucose-fueled mitochondrial oxidative phosphorylation and defective hematopoietic stem cells.

Li J, Bai J, Pham V, Hashimoto M, Sezaki M, Shi Q J Clin Invest. 2025; 135(5).

PMID: 39786963 PMC: 11870731. DOI: 10.1172/JCI184069.

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