Therapeutic Potential of RS3-resistant Starch in Alleviating Neuroinflammation and Apoptosis in a Parkinson's Disease Rat Model

Overview

Journal Heliyon

Specialty Social Sciences

Date 2024 Sep 30

PMID 39347419

Authors

Qian-Kun He

Xue-Yong Wang

Wei Hu

Jing Cai

Peng Chen

Ming-Wei Liu

Yuan-Hua Wu

Affiliations

Soon will be listed here.

Abstract

This study aimed to investigate the effects of Miao medicinal RS3-resistant starch on behavioral performance and substantia nigra neuron apoptosis-related indicators in a rat model of Parkinson's disease (PD). Among the experimental groups, except for the control group, we induced PD rat models by subcutaneous injection of rotenone in the neck and back. After model induction, a 28-day drug intervention was conducted. Various techniques have been employed, including behavioral analysis, Real-time Polymerase Chain Reaction (RT-PCR), western blotting, enzyme-linked immunosorbent assay (ELISA), immunofluorescence, and terminal deoxynucleotidyltransferase-mediated UTP nick-ends. labeling (TUNEL) and Nissl staining to investigate the effect of RS3-resistant starch on the substantia nigra and neuronal apoptosis-related markers in the brains of PD model rats. Our study revealed that RS3, a resistant starch, significantly reduced the climbing time of PD model rats, prolonged their hanging time, lowered the expression levels of the inflammatory factors IL-1β, IL-6, and TNF-α, increased the number of TH-positive neurons in the substantia nigra, and decreased the levels of IL-1β, IL-6, and TNF-α. Furthermore, RS3 elevated the protein expression levels of tyrosine hydroxylase (TH) and Bcl-2 while reducing those of Bax, TLR4, NLRP3,and p-P65, and mitigated apoptosis and morphological changes in dopaminergic neurons in the substantia nigra region. Our results suggest that RS3-resistant starch may offer therapeutic benefits for PD patients with PD by potentially influencing inflammation and apoptosis in the dopaminergic system.

References

Heidari A, Yazdanpanah N, Rezaei N . The role of Toll-like receptors and neuroinflammation in Parkinson's disease. J Neuroinflammation. 2022; 19(1):135. PMC: 9172200. DOI: 10.1186/s12974-022-02496-w. View

Agarwal R, Sarma S . Restoring the basal ganglia in Parkinson's disease to normal via multi-input phase-shifted deep brain stimulation. Annu Int Conf IEEE Eng Med Biol Soc. 2010; 2010:1539-42. PMC: 4107297. DOI: 10.1109/IEMBS.2010.5626828. View

Bachhuber T, Katzmarski N, McCarter J, Loreth D, Tahirovic S, Kamp F . Inhibition of amyloid-β plaque formation by α-synuclein. Nat Med. 2015; 21(7):802-7. DOI: 10.1038/nm.3885. View

Wu J, Qiu M, Zhang C, Zhang C, Wang N, Zhao F . Type 3 resistant starch from modulates obesity and obesity-related low-grade systemic inflammation in mice by regulating gut microbiota composition and metabolism. Food Funct. 2021; 12(23):12098-12114. DOI: 10.1039/d1fo02208c. View

Freeman L, Ting J . The pathogenic role of the inflammasome in neurodegenerative diseases. J Neurochem. 2015; 136 Suppl 1:29-38. DOI: 10.1111/jnc.13217. View

Zhang N, Dou D, Ran X, Kang T . Neuroprotective effect of arctigenin against neuroinflammation and oxidative stress induced by rotenone. RSC Adv. 2022; 8(5):2280-2292. PMC: 9077403. DOI: 10.1039/c7ra10906g. View

Araujo B, Caridade-Silva R, Soares-Guedes C, Martins-Macedo J, Gomes E, Monteiro S . Neuroinflammation and Parkinson's Disease-From Neurodegeneration to Therapeutic Opportunities. Cells. 2022; 11(18). PMC: 9497016. DOI: 10.3390/cells11182908. View

Zhang J, Wang Z, Shi X . Canna edulis Ker by-product: chemical composition and characteristics of the dietary fiber. Food Sci Technol Int. 2011; 16(4):305-13. DOI: 10.1177/1082013209353832. View

Zhang J, Zhang Z, Bao J, Yu Z, Cai M, Li X . Jia-Jian-Di-Huang-Yin-Zi decoction reduces apoptosis induced by both mitochondrial and endoplasmic reticulum caspase12 pathways in the mouse model of Parkinson's disease. J Ethnopharmacol. 2017; 203:69-79. DOI: 10.1016/j.jep.2016.12.053. View

10.

Zhang C, Ma S, Wu J, Luo L, Qiao S, Li R . A specific gut microbiota and metabolomic profiles shifts related to antidiabetic action: The similar and complementary antidiabetic properties of type 3 resistant starch from Canna edulis and metformin. Pharmacol Res. 2020; 159:104985. DOI: 10.1016/j.phrs.2020.104985. View

11.

. Global, regional, and national burden of neurological disorders, 1990-2016: a systematic analysis for the Global Burden of Disease Study 2016. Lancet Neurol. 2019; 18(5):459-480. PMC: 6459001. DOI: 10.1016/S1474-4422(18)30499-X. View

12.

Ye Y, Jin T, Zhang X, Zeng Z, Ye B, Wang J . Meisoindigo Protects Against Focal Cerebral Ischemia-Reperfusion Injury by Inhibiting NLRP3 Inflammasome Activation and Regulating Microglia/Macrophage Polarization via TLR4/NF-κB Signaling Pathway. Front Cell Neurosci. 2020; 13:553. PMC: 6930809. DOI: 10.3389/fncel.2019.00553. View

13.

Codolo G, Plotegher N, Pozzobon T, Brucale M, Tessari I, Bubacco L . Triggering of inflammasome by aggregated α-synuclein, an inflammatory response in synucleinopathies. PLoS One. 2013; 8(1):e55375. PMC: 3561263. DOI: 10.1371/journal.pone.0055375. View

14.

Otari S, Pawar S, Patel S, Singh R, Kim S, Lee J . Leaf Extract-Mediated Preparation of Stabilized Silver Nanoparticles: Characterization, Antimicrobial Activity, and Toxicity Studies. J Microbiol Biotechnol. 2017; 27(4):731-738. DOI: 10.4014/jmb.1610.10019. View

15.

Makdissi S, Parsons B, Di Cara F . Towards early detection of neurodegenerative diseases: A gut feeling. Front Cell Dev Biol. 2023; 11:1087091. PMC: 9941184. DOI: 10.3389/fcell.2023.1087091. View

16.

Jing X, Yang H, Taximaimaiti R, Wang X . Advances in the Therapeutic Use of Non-Ergot Dopamine Agonists in the Treatment of Motor and Non-Motor Symptoms of Parkinson's Disease. Curr Neuropharmacol. 2022; 21(5):1224-1240. PMC: 10286583. DOI: 10.2174/1570159X20666220915091022. View

17.

Zhao Y, Qiong-Zhang , Zhang J, Lou Z, Zu H, Wang Z . The Synergy of Aging and LPS Exposure in a Mouse Model of Parkinson's Disease. Aging Dis. 2018; 9(5):785-797. PMC: 6147589. DOI: 10.14336/AD.2017.1028. View

18.

Reeve A, Ludtmann M, Angelova P, Simcox E, Horrocks M, Klenerman D . Aggregated α-synuclein and complex I deficiency: exploration of their relationship in differentiated neurons. Cell Death Dis. 2015; 6:e1820. PMC: 4650719. DOI: 10.1038/cddis.2015.166. View

19.

Han L, Xie Y, Wu R, Chen C, Zhang Y, Wang X . Traditional Chinese medicine for modern treatment of Parkinson's disease. Chin J Integr Med. 2017; 23(8):635-640. DOI: 10.1007/s11655-016-2537-7. View

20.

Li Y, Xia Y, Yin S, Wan F, Hu J, Kou L . Targeting Microglial α-Synuclein/TLRs/NF-kappaB/NLRP3 Inflammasome Axis in Parkinson's Disease. Front Immunol. 2021; 12:719807. PMC: 8531525. DOI: 10.3389/fimmu.2021.719807. View