Exploring the Tolerable Region for HiBiT Tag Insertion in the Hepatitis B Virus Genome

Overview

Journal mSphere

Publisher American Society for Microbiology

Specialties Microbiology
Parasitology

Date 2024 Sep 30

PMID 39345122

Authors

Asako Murayama

Hitomi Igarashi

Norie Yamada

Hussein Hassan Aly

Masaaki Toyama

Masanori Isogawa

Tetsuro Shimakami

Takanobu Kato

Affiliations

Soon will be listed here.

Abstract

Importance: Hepatitis B virus (HBV) is the principal causative agent of chronic hepatitis. Despite the availability of vaccines in many countries, HBV infection has spread worldwide and caused chronic infection. In chronic hepatitis B patients, liver inflammation leads to cirrhosis, and the accumulation of viral genome integration into host chromosomes leads to the development of hepatocellular carcinoma. The currently available treatment strategy cannot expect the eradication of HBV. To explore novel anti-HBV agents, a cell culture system that can detect HBV infection easily is indispensable. In this study, we examined the regions in the HBV genome where the high affinity and bright luminescence (HiBiT) tag could be inserted and established an HBV infection system to monitor infection by measuring the HiBiT signal by infecting the HiBiT-tagged HBV in sodium taurocholate cotransporting polypeptide-transduced HepG2 (HepG2/NTCP) cells. This system can contribute to screening for novel anti-HBV agents.

Citing Articles

Generation of Replication-Competent Hepatitis B Virus Harboring Tagged Polymerase for Visualization and Quantification of the Infection.

Morita C, Wada M, Ohsaki E, Kimura-Ohba S, Ueda K Microbiol Immunol. 2024; 69(1):43-58.

PMID: 39620377 PMC: 11701411. DOI: 10.1111/1348-0421.13183.

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