New Viruses Infecting Hyperthermophilic Bacterium

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2024 Sep 28

PMID 39339886

Authors

Matvey Kolesnik

Constantine Pavlov

Alina Demkina

Aleksei Samolygo

Karyna Karneyeva

Anna Trofimova

Olga S Sokolova

Andrei V Moiseenko

Maria Kirsanova

Konstantin Severinov

Affiliations

Soon will be listed here.

Abstract

Highly diverse phages infecting thermophilic bacteria of the genus have been isolated over the years from hot springs around the world. Many of these phages are unique, rely on highly unusual developmental strategies, and encode novel enzymes. The variety of phages is clearly undersampled, as evidenced, for example, by a paucity of phage-matching spacers in CRISPR arrays. Using water samples collected from hot springs in the Kunashir Island from the Kuril archipelago and from the Tsaishi and Nokalakevi districts in the Republic of Georgia, we isolated several distinct phages infecting laboratory strains of . Genomic sequence analysis of 11 phages revealed both close relatives of previously described phages isolated from geographically distant sites, as well as phages with very limited similarity to earlier isolates. Comparative analysis allowed us to predict several accessory phage genes whose products may be involved in host defense/interviral warfare, including a putative Type V CRISPR- system.

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