Article: Genomic Sequence of a Czech Isolate of Erysimum Latent Virus from

The Erysimum latent virus (ELV), a tymovirus, was first isolated from several wild and cultivated brassicas in Germany. Its virions were shown to be serologically distinct from those of the turnip yellow mosaic virus (TYMV), which is also found in wild and cultivated plants in several European countries but also in other parts of the world. TYMV and ELV were among the first plant viruses to have had their genomes sequenced, and when other tymovirus genomes were sequenced, it was found that, in phylogenies, ELV is probably the basal outlier to all other tymoviruses. Here, we report the near-complete genomic sequence of another isolate of ELV from Czechia. This isolate was found in 1990 in plants showing mosaic symptoms. It was detected using ELISA tests and electron microscopy. We have now sequenced the full coding sequence of this isolate using contemporary high throughput methods and found that the German and Czech isolates of ELV are closely related and are of the same virus species.

Citing Articles

Plant Viruses, Viroids and Phytoplasmas: Insight into Evolutionary, Pathogenicity, and Epidemiology Studies.

Ferretti L, Taglienti A Plants (Basel). 2025; 14(4).

PMID: 40006810 PMC: 11858912. DOI: 10.3390/plants14040551.

References

1.

Rollin J, Bester R, Brostaux Y, Caglayan K, De Jonghe K, Eichmeier A . Detection of single nucleotide polymorphisms in virus genomes assembled from high-throughput sequencing data: large-scale performance testing of sequence analysis strategies. PeerJ. 2023; 11:e15816. PMC: 10439718. DOI: 10.7717/peerj.15816. View

2.

Ding S, Howe J, Keese P, MacKenzie A, Meek D, Skotnicki M . The tymobox, a sequence shared by most tymoviruses: its use in molecular studies of tymoviruses. Nucleic Acids Res. 1990; 18(5):1181-7. PMC: 330433. DOI: 10.1093/nar/18.5.1181. View

3.

Bozarth C, Weiland J, Dreher T . Expression of ORF-69 of turnip yellow mosaic virus is necessary for viral spread in plants. Virology. 1992; 187(1):124-30. DOI: 10.1016/0042-6822(92)90301-5. View

4.

Srifah P, Keese P, Shukla D, Gibbs A . The primary structure of the virion protein gene and encoded protein of erysimum latent tymovirus. J Gen Virol. 1990; 71 ( Pt 12):3085-8. DOI: 10.1099/0022-1317-71-12-3085. View

5.

Saldarelli P, Giampetruzzi A, Morelli M, Malossini U, Pirolo C, Bianchedi P . Genetic Variability of Grapevine Pinot gris virus and Its Association with Grapevine Leaf Mottling and Deformation. Phytopathology. 2014; 105(4):555-63. DOI: 10.1094/PHYTO-09-14-0241-R. View

6.

Martin D, Varsani A, Roumagnac P, Botha G, Maslamoney S, Schwab T . RDP5: a computer program for analyzing recombination in, and removing signals of recombination from, nucleotide sequence datasets. Virus Evol. 2021; 7(1):veaa087. PMC: 8062008. DOI: 10.1093/ve/veaa087. View

7.

Tamura K, Stecher G, Kumar S . MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Mol Biol Evol. 2021; 38(7):3022-3027. PMC: 8233496. DOI: 10.1093/molbev/msab120. View

8.

Tsai C, Dreher T . Increased viral yield and symptom severity result from a single amino acid substitution in the turnip yellow mosaic virus movement protein. Mol Plant Microbe Interact. 1993; 6(3):268-73. DOI: 10.1094/mpmi-6-268. View

9.

Srifah P, Keese P, Weiller G, Gibbs A . Comparisons of the genomic sequences of erysimum latent virus and other tymoviruses: a search for the molecular basis of their host specificities. J Gen Virol. 1992; 73 ( Pt 6):1437-47. DOI: 10.1099/0022-1317-73-6-1437. View

10.

Glasa M, Predajna L, Kominek P, Nagyova A, Candresse T, Olmos A . Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines. Arch Virol. 2014; 159(8):2103-7. DOI: 10.1007/s00705-014-2031-5. View