Thermodynamic Study on Biomimetic Bacterial Membranes

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2024 Sep 28

PMID 39339363

Authors

Katarzyna Pastuszak

Marta Palusinska-Szysz

Agnieszka Ewa Wiacek

Malgorzata Jurak

Affiliations

Soon will be listed here.

Abstract

The presented studies were aimed at determining the interactions in model membranes (Langmuir monolayers) created of phospholipids (PL) isolated from bacteria cultured with (PL + choline) or without (PL - choline) choline and to describe the impact of an antimicrobial peptide, human cathelicidin LL-37, on PL's monolayer behavior. The addition of choline to the growth medium influenced the mutual proportions of phospholipids extracted from . Four classes of phospholipids-phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), cardiolipin (CL), and their mixtures-were used to register compression isotherms with or without the LL-37 peptide in the subphase. Based on them the excess area (Ae), excess (ΔGe), and total (ΔGm) Gibbs energy of mixing were determined. The thermodynamic analyses revealed that the PL - choline monolayer showed greater repulsive forces between molecules in comparison to the ideal system, while the PL + choline monolayer was characterized by greater attraction. The LL-37 peptide affected the strength of interactions between phospholipids' molecules and reduced the monolayers stability. Accordingly, the changes in interactions in the model membranes allowed us to determine the difference in their susceptibility to the LL-37 peptide depending on the choline supplementation of bacterial culture.

References

Campbell R, Saaka Y, Shao Y, Gerelli Y, Cubitt R, Nazaruk E . Structure of surfactant and phospholipid monolayers at the air/water interface modeled from neutron reflectivity data. J Colloid Interface Sci. 2018; 531:98-108. DOI: 10.1016/j.jcis.2018.07.022. View

Neville F, Cahuzac M, Konovalov O, Ishitsuka Y, Lee K, Kuzmenko I . Lipid headgroup discrimination by antimicrobial peptide LL-37: insight into mechanism of action. Biophys J. 2005; 90(4):1275-87. PMC: 1367279. DOI: 10.1529/biophysj.105.067595. View

. The monolayer technique: a potent tool for studying the interfacial properties of antimicrobial and membrane-lytic peptides and their interactions with lipid membranes. Biochim Biophys Acta. 1999; 1462(1-2):109-40. DOI: 10.1016/s0005-2736(99)00203-5. View

Palusinska-Szysz M, Szuster-Ciesielska A, Janczarek M, Wdowiak-Wrobel S, Schiller J, Reszczynska E . Genetic diversity of Legionella pcs and pmtA genes and the effect of utilization of choline by Legionella spp. on induction of proinflammatory cytokines. Pathog Dis. 2019; 77(7). DOI: 10.1093/femspd/ftz065. View

Zhao L, Cao Z, Bian Y, Hu G, Wang J, Zhou Y . Molecular Dynamics Simulations of Human Antimicrobial Peptide LL-37 in Model POPC and POPG Lipid Bilayers. Int J Mol Sci. 2018; 19(4). PMC: 5979298. DOI: 10.3390/ijms19041186. View

Pastuszak K, Jurak M, Kowalczyk B, Tarasiuk J, Wiacek A, Palusinska-Szysz M . Susceptibility of Membrane-Derived Phospholipids to the Peptide Action of Antimicrobial LL-37-Langmuir Monolayer Studies. Molecules. 2024; 29(7). PMC: 11013288. DOI: 10.3390/molecules29071522. View

Wang J, Wang J, Wang X, Wang Z . Effect of Amphotericin B on the Thermodynamic Properties and Surface Morphology of the Pulmonary Surfactant Model Monolayer during Respiration. Molecules. 2023; 28(12). PMC: 10305212. DOI: 10.3390/molecules28124840. View

Zhao H, Lappalainen P . A simple guide to biochemical approaches for analyzing protein-lipid interactions. Mol Biol Cell. 2012; 23(15):2823-30. PMC: 3408410. DOI: 10.1091/mbc.E11-07-0645. View

Pastuszak K, Chmiel E, Kowalczyk B, Tarasiuk J, Jurak M, Palusinska-Szysz M . Physicochemical Characteristics of Model Membranes Composed of Lipids. Membranes (Basel). 2023; 13(3). PMC: 10058700. DOI: 10.3390/membranes13030356. View

10.

BLIGH E, Dyer W . A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959; 37(8):911-7. DOI: 10.1139/o59-099. View

11.

Pastuszak K, Kowalczyk B, Tarasiuk J, Luchowski R, Gruszecki W, Jurak M . Insight into the Mechanism of Interactions between the LL-37 Peptide and Model Membranes of Bacteria. Int J Mol Sci. 2023; 24(15). PMC: 10418352. DOI: 10.3390/ijms241512039. View

12.

Thennarasu S, Tan A, Penumatchu R, Shelburne C, Heyl D, Ramamoorthy A . Antimicrobial and membrane disrupting activities of a peptide derived from the human cathelicidin antimicrobial peptide LL37. Biophys J. 2010; 98(2):248-57. PMC: 2808482. DOI: 10.1016/j.bpj.2009.09.060. View

13.

He M, Ding N . Plant Unsaturated Fatty Acids: Multiple Roles in Stress Response. Front Plant Sci. 2020; 11:562785. PMC: 7500430. DOI: 10.3389/fpls.2020.562785. View

14.

Chauhan D, Shames S . Pathogenicity and Virulence of : Intracellular replication and host response. Virulence. 2021; 12(1):1122-1144. PMC: 8043192. DOI: 10.1080/21505594.2021.1903199. View

15.

Ephros M, Engelhard D, Maayan S, Bercovier H, Avital A, Yatsiv I . Legionella gormanii pneumonia in a child with chronic granulomatous disease. Pediatr Infect Dis J. 1989; 8(10):726-7. View

16.

Jurak M, Mroczka R, Lopucki R . Properties of Artificial Phospholipid Membranes Containing Lauryl Gallate or Cholesterol. J Membr Biol. 2018; 251(2):277-294. PMC: 5910520. DOI: 10.1007/s00232-018-0025-z. View

17.

Qin T, Zhou H, Ren H, Liu W . Distribution of Secretion Systems in the Genus and Its Correlation with Pathogenicity. Front Microbiol. 2017; 8:388. PMC: 5348487. DOI: 10.3389/fmicb.2017.00388. View

18.

Wilson B, Ramanathan A, Lopez C . Cardiolipin-Dependent Properties of Model Mitochondrial Membranes from Molecular Simulations. Biophys J. 2019; 117(3):429-444. PMC: 6697365. DOI: 10.1016/j.bpj.2019.06.023. View

19.

Wydro P, Witkowska K . The interactions between phosphatidylglycerol and phosphatidylethanolamines in model bacterial membranes: the effect of the acyl chain length and saturation. Colloids Surf B Biointerfaces. 2009; 72(1):32-9. DOI: 10.1016/j.colsurfb.2009.03.011. View

20.

van Meer G, Voelker D, Feigenson G . Membrane lipids: where they are and how they behave. Nat Rev Mol Cell Biol. 2008; 9(2):112-24. PMC: 2642958. DOI: 10.1038/nrm2330. View