Metabolomic and Physiological Analyses Reveal the Effects of Different Storage Conditions on Hu Seeds

Overview

Journal Metabolites

Publisher MDPI

Date 2024 Sep 27

PMID 39330510

Authors

Hao Cai

Yongbao Shen

Affiliations

Soon will be listed here.

Abstract

Backgrounds: Hu is a deciduous tree in the family, and it is classified as a Class II endangered plant in China. Seed storage technology is an effective means of conserving germplasm resources, but the effects of different storage conditions on the quality and associated metabolism of seeds remain unclear. This study analyzed the physiological and metabolic characteristics of seeds under four storage conditions.

Results: Our findings demonstrate that reducing seed moisture content and storage temperature effectively prolongs storage life. Seeds stored under that condition exhibited higher internal nutrient levels, lower endogenous abscisic acid (ABA) hormone levels, and elevated gibberellic acid (GA) levels. Additionally, 335 metabolites were identified under four different storage conditions. The analysis indicates that seeds extend seed longevity and maintain cellular structural stability mainly by regulating the changes in metabolites related to lipid, amino acid, carbohydrate, and carotenoid metabolic pathways under the storage conditions of a low temperature and low seed moisture.

Conclusions: These findings provide new insights at the physiological and metabolic levels into how these storage conditions extend seed longevity while also offering effective storage strategies for preserving the germplasm resources of .

Citing Articles

Cooking Alters the Metabolites of Onions and Their Ability to Protect Nerve Cells from Lead Damage.

Zhao L, Wang L, Wang N, Gao X, Zhang B, Zhao Y Foods. 2024; 13(22).

PMID: 39594122 PMC: 11593875. DOI: 10.3390/foods13223707.

References

He M, Ding N . Plant Unsaturated Fatty Acids: Multiple Roles in Stress Response. Front Plant Sci. 2020; 11:562785. PMC: 7500430. DOI: 10.3389/fpls.2020.562785. View

Zhuang X, Zhang Y, Xiao A, Zhang A, Fang B . Key Enzymes in Fatty Acid Synthesis Pathway for Bioactive Lipids Biosynthesis. Front Nutr. 2022; 9:851402. PMC: 8905437. DOI: 10.3389/fnut.2022.851402. View

Hussain S, Zheng M, Khan F, Khaliq A, Fahad S, Peng S . Benefits of rice seed priming are offset permanently by prolonged storage and the storage conditions. Sci Rep. 2015; 5:8101. PMC: 4309961. DOI: 10.1038/srep08101. View

Ahmad N, Malagoli M, Wirtz M, Hell R . Drought stress in maize causes differential acclimation responses of glutathione and sulfur metabolism in leaves and roots. BMC Plant Biol. 2016; 16(1):247. PMC: 5103438. DOI: 10.1186/s12870-016-0940-z. View

Sonnweber T, Pizzini A, Nairz M, Weiss G, Tancevski I . Arachidonic Acid Metabolites in Cardiovascular and Metabolic Diseases. Int J Mol Sci. 2018; 19(11). PMC: 6274989. DOI: 10.3390/ijms19113285. View

Figueroa C, Lunn J, Iglesias A . Nucleotide-sugar metabolism in plants: the legacy of Luis F. Leloir. J Exp Bot. 2021; 72(11):4053-4067. DOI: 10.1093/jxb/erab109. View

Schaller A, Stintzi A . Enzymes in jasmonate biosynthesis - structure, function, regulation. Phytochemistry. 2009; 70(13-14):1532-8. DOI: 10.1016/j.phytochem.2009.07.032. View

Zhou W, Chen F, Luo X, Dai Y, Yang Y, Zheng C . A matter of life and death: Molecular, physiological, and environmental regulation of seed longevity. Plant Cell Environ. 2019; 43(2):293-302. DOI: 10.1111/pce.13666. View

Shanab S, Hafez R, Fouad A . A review on algae and plants as potential source of arachidonic acid. J Adv Res. 2018; 11:3-13. PMC: 6052662. DOI: 10.1016/j.jare.2018.03.004. View

10.

Wahab A, Abdi G, Saleem M, Ali B, Ullah S, Shah W . Plants' Physio-Biochemical and Phyto-Hormonal Responses to Alleviate the Adverse Effects of Drought Stress: A Comprehensive Review. Plants (Basel). 2022; 11(13). PMC: 9269229. DOI: 10.3390/plants11131620. View

11.

Dong Y, Wang X, Ahmad N, Sun Y, Wang Y, Liu X . The Carthamus tinctorius L. genome sequence provides insights into synthesis of unsaturated fatty acids. BMC Genomics. 2024; 25(1):510. PMC: 11112859. DOI: 10.1186/s12864-024-10405-z. View

12.

Murthy U, Sun W . Protein modification by Amadori and Maillard reactions during seed storage: roles of sugar hydrolysis and lipid peroxidation. J Exp Bot. 2000; 51(348):1221-8. DOI: 10.1093/jexbot/51.348.1221. View

13.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

14.

Luque de Castro M, Priego-Capote F . Soxhlet extraction: Past and present panacea. J Chromatogr A. 2009; 1217(16):2383-9. DOI: 10.1016/j.chroma.2009.11.027. View

15.

Walters C, Berjak P, Pammenter N, Kennedy K, Raven P . Plant science. Preservation of recalcitrant seeds. Science. 2013; 339(6122):915-6. DOI: 10.1126/science.1230935. View

16.

Wang Y, Li B, Du M, Eneji A, Wang B, Duan L . Mechanism of phytohormone involvement in feedback regulation of cotton leaf senescence induced by potassium deficiency. J Exp Bot. 2012; 63(16):5887-901. PMC: 3467299. DOI: 10.1093/jxb/ers238. View

17.

Zhu S, Wei X, Lu Y, Zhang D, Wang Z, Ge J . The jacktree genome and population genomics provides insights for the mechanisms of the germination obstacle and the conservation of endangered ornamental plants. Hortic Res. 2024; 11(8):uhae166. PMC: 11300842. DOI: 10.1093/hr/uhae166. View

18.

Yan S, Huang W, Gao J, Fu H, Liu J . Comparative metabolomic analysis of seed metabolites associated with seed storability in rice (Oryza sativa L.) during natural aging. Plant Physiol Biochem. 2018; 127:590-598. DOI: 10.1016/j.plaphy.2018.04.020. View

19.

Solberg S, Yndgaard F, Andreasen C, von Bothmer R, Loskutov I, Asdal A . Long-Term Storage and Longevity of Orthodox Seeds: A Systematic Review. Front Plant Sci. 2020; 11:1007. PMC: 7347988. DOI: 10.3389/fpls.2020.01007. View

20.

Attri P, Ishikawa K, Okumura T, Koga K, Shiratani M, Mildaziene V . Impact of seed color and storage time on the radish seed germination and sprout growth in plasma agriculture. Sci Rep. 2021; 11(1):2539. PMC: 7844220. DOI: 10.1038/s41598-021-81175-x. View