Effects of Ergothioneine Supplementation on Meiotic Competence and Porcine Oocyte Development

Overview

Journal Vet World

Specialty Veterinary Medicine

Date 2024 Sep 27

PMID 39328438

Authors

Megumi Nagahara

Zhao Namula

Qingyi Lin

Koki Takebayashi

Nanaka Torigoe

Bin Liu

Fuminori Tanihara

Takeshige Otoi

Maki Hirata

Affiliations

Soon will be listed here.

Abstract

Background And Aim: The antioxidant effects of ergothioneine (EGT) on culture of porcine zygote are not well established. The study investigated the impact of EGT supplementation on meiotic competence and development of porcine oocytes.

Materials And Methods: The impact of EGT concentrations (0, 5, 10, 25, 50, and 100 μM) during maturation (IVM) on the progression of meiotic maturation, fertilization, and blastocyst formation in porcine oocytes was assessed. The DNA fragmentation level was evaluated to determine oocyte and blastocyst quality.

Results: The proportion of metaphase II oocytes was significantly greater (p < 0.05) in EGT-cultured oocytes than in control oocytes, regardless of the EGT concentration, and those oocytes with 10 μM or more EGT had fewer DNA-fragmented nuclei (p < 0.05). Blastocysts derived from oocytes cultured with 10 μM EGT had the highest proportion (p < 0.05), while those from control oocytes or oocytes cultured with 50 μM or less EGT had significantly higher proportions. Despite EGT supplementation, there were no noticeable differences in total cell numbers and DNA fragmentation indices in the derived blastocysts.

Conclusion: Supplementing with EGT during IVM leads to better oocyte maturation, quality, and embryonic development due to decreased DNA fragmentation. The present study failed to elucidate the mechanism of DNA fragmentation reduction by EGT. More research needs to be conducted to explore the antioxidant mechanism of EGT.

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