Diverse Engraftment Capability of Neuroblastoma Cell Lines in Zebrafish Larvae

Overview

Journal Zebrafish

Date 2024 Sep 24

PMID 39316469

Authors

Josephine M Lawrence

Shyang Hong Tan

Daniel C Kim

Ke-En Tan

Sydney E Schroeder

Kok Siong Yeo

Madison A Schaefer

Alexis M Sosic

Shizhen Zhu

Affiliations

Soon will be listed here.

Abstract

Xenotransplantation of neuroblastoma cells into larval zebrafish allows the characterization of their tumorigenic abilities and high-throughput treatment screening. This established preclinical model traditionally relies on microinjection into the yolk or perivitelline space, leaving the engraftment ability of cells at the hindbrain ventricle (HBV) and pericardial space (PCS), sites valuable for evaluating metastasis, angiogenesis, and the brain microenvironment, unknown. To address this gap in knowledge, Casper zebrafish at 48 h postfertilization were microinjected with approximately 200 Kelly, Be(2)-C, SK-N-AS, or SY5Y cells into either the HBV or PCS. Fish were imaged at 1, 3, and 6 days postinjection and tumor growth was monitored at each timepoint. We hypothesized that engraftment ability and location preference would be cell line dependent. Kelly and SK-N-AS cells were able to engraft at both the HBV and PCS, with a near doubling in size of tumor volume during the 6 days observation period, with cells appearing to grow better in the HBV. Be(2)-C tumors remained static while SY5Y tumors decreased in size, with almost complete loss of volume at both sites. Therefore, the capability of neuroblastoma cell engraftment in zebrafish larvae is cell line dependent with a location preference.

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